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Mutational analysis of two Arabidopsis thaliana cyclin-dependent kinases in fission yeast

Porceddu, Andrea and De Veyldera, Lieven and Hayles, Jacky and Van Montagu, Marc and Inzé, Dirk and Mironov, Vladimir A. (1999) Mutational analysis of two Arabidopsis thaliana cyclin-dependent kinases in fission yeast. FEBS Letters, Vol. 446 (1), p. 182-188. ISSN 0014-5793. Article.

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DOI: 10.1016/S0014-5793(99)00211-2


We have analyzed five mutant alleles of two cyclin-dependent kinases from Arabidopsis thaliana, CDC2aAt and CDC2bAt, in Schizosaccharomyces pombe. Two of the five mutant alleles produced similar phenotypes for both cyclin-dependent kinases. The other three mutants caused phenotypes dependent on the particular cyclin-dependent kinase. Of all the mutant alleles, only two were found to possess a detectable kinase activity. Our mutational analysis lends further support for CDC2aAt being the true orthologue of the yeast cdc2. CDC2bAt, even though quite divergent from S. pombe cdc2, still retains the ability to interact with at least some essential cell cycle regulators, suggesting some functional homology with the yeast protein. Additionally, we demonstrated that the three amino acid deletion in the DL50 mutants results in the loss of the ability to interact with the suc1/CKS1 proteins.

Item Type:Article
ID Code:4186
Uncontrolled Keywords:Cell cycle, cyclin-dependent kinase, CDC2 kinase subunit, Arabidopsis thaliana, Schizosaccharomyces pombe
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/07 Genetica agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze agronomiche e genetica vegetale agraria
Copyright Holders:© 1999 Federation of European Biochemical Societies
Additional Information:Erratum to: `Mutational analysis of two Arabidopsis thaliana cyclin-dependent kinases in fission yeast' pubblicato su FEBS Letters, 2009, vol. 454, 1-2, p. 172.
Deposited On:15 Jul 2010 09:25

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