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Detection of globin chains by reversed-phase high-performance liquid chromatography

Masala, Bruno Lucio and Manca, Laura (1994) Detection of globin chains by reversed-phase high-performance liquid chromatography. Methods in Enzymology, Vol. 231 , p. 21-44. ISSN 0076-6879. Article.

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DOI: 10.1016/0076-6879(94)31005-X

Abstract

Reversed-phase high-performance liquid chromatography (RP-HPLC) has greatly influenced and stimulated research in detection, quantitation, and identification of normal and abnormal globin chains in human newborn and adult red blood cells. In fact, RP-HPLC methodology has a number of advantages: it is fast and accurate, minute amounts of material are sufficient for each determination, complete automation is possible, and, most importantly, identification of electrophoretically "silent" mutant hemoglobins due to neutral-to-neutral amino acid substitutions is feasible and reliable. It also greatly facilitates the separation and isolation of even microquantities of a mutant globin chain, and of globin proteolytic fragments, for subsequent characterization of the substitution within the abnormal peptide.

Item Type:Article
ID Code:4179
Status:Published
Refereed:Yes
Uncontrolled Keywords:Polyacrylamide-gel electrophoresis, Bubalus-bubalis L., a-gamma-globin, hemoglobin chains, alpha-globin, mass-spectrometry, gene arrangements, tryptic peptides, fetal hemoglobin, newborn babies
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Academic Press / Elsevier
ISSN:0076-6879
Additional Information:Fa parte di: Johannes Everse, Kim D. Vandegriff, Robert M. Winslow, Editor(s), Methods in Enzymology, Academic Press, 1994, Volume 231, Hemoglobins Part B: Biochemical and Analytical Methods, Pages 21-44, ISBN 9780121821326.
Deposited On:15 Jul 2010 11:33

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