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Transgenic plants expressing human glutamic acid decarboxylase (GAD65), a major autoantigen in insulin-dependent diabetes mellitus

Porceddu, Andrea and Falorni, Alberto and Ferradini, Nicoletta and Cosentino, Anna and Calcinaro, Filippo and Faleri, Claudia and Cresti, Mauro and Lorenzetti, Franco and Brunetti, Paolo and Pezzotti, Mario (1999) Transgenic plants expressing human glutamic acid decarboxylase (GAD65), a major autoantigen in insulin-dependent diabetes mellitus. Molecular Breeding, Vol. 5 (6), p. 553-560. eISSN 1572-9788. Article.

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DOI: 10.1023/A:1009605729268


Parenteral and oral administration of autoantigens can induce immune tolerance in autoimmune diseases. Prophylactic therapy based on oral administration of human autoantigens is not, however, feasible when sufficient quantities of candidate autoantigens are not available. Transgenic plants that express high levels of recombinant proteins would allow large quantities of autoantigens to be produced at relatively low costs. In addition, transgenic food would provide a simple and direct method of delivering autoantigens. The production and the characterization of transgenic tobacco and carrot plants expressing human GAD65, a major autoantigen in human insulin-dependent diabetes mellitus (IDDM), is reported. Immunogold labeling and electron microscopy of transgenic tobacco tissue shows the selective targeting of human GAD65 to chloroplast tylacoids and mitochondria. In planta expressed GAD65 has a correct immunoreactivity with IDDM-associated autoantibodies and retains enzymatic activity, a finding that suggests a correct protein folding. In transgenic tobacco and carrot the expression levels of human GAD65 varies between 0.01%and 0.04%of total soluble proteins. Transgenic edible plant organs are nowavailable to study the feasibility of inducing immune tolerance in IDDM animals by oral administration of GAD65.

Item Type:Article
ID Code:4150
Uncontrolled Keywords:Autoimmunity, carrot, diabetes mellitus, oral tolerance, tobacco, transgenic plants
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/07 Genetica agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze agronomiche e genetica vegetale agraria
Publisher:Kluwer / Springer
Copyright Holders:© 1999 Kluwer Academic Publishers
Deposited On:12 Jul 2010 10:48

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