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Microsatellite markers are powerful tools for discriminating among olive cultivars and assigning them to geographically defined populations

Sarri, Vania and Baldoni, Luciana and Porceddu, Andrea and Cultrera, N.G.M. and Contento, A. and Frediani, M. and Belaj, Angjelina and Trujillo Navas, Isabel and Cionini, P.G. (2006) Microsatellite markers are powerful tools for discriminating among olive cultivars and assigning them to geographically defined populations. Genome, Vol. 49 (12), p. 1606-1615. eISSN 1480-3321. Article.

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DOI: 10.1139/G06-126

Abstract

Twelve simple sequence repeat (SSR) loci were used to differentiate among 118 cultivars sampled in several countries of the Mediterranean basin and to analyze the genetic structure of olive cultivar gene pools. The markers were found to have high discrimination power. On average, with a single assay it was possible to discriminate 96% of the pairwise comparisons and, with a combination of 3 loci, virtually all cultivars were distinguished. The SSR markers were also tested for their ability to assign cultivars to their geographic population of origin. A selection of 6 loci was found to maximize assignment accuracy, correctly reallocating up to 75.4% of cultivars to their population of origin. Because of the confusion surrounding the origin of most olive cultivars, their molecular identification and ascertainment of origin will be extremely useful for germplasm management and breeding.

Item Type:Article
ID Code:4146
Status:Published
Refereed:Yes
Uncontrolled Keywords:SSR, Olea europaea, cultivar discrimination, assignment test, private alleles
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/07 Genetica agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze agronomiche e genetica vegetale agraria
Publisher:NRC Research Press
eISSN:1480-3321
Copyright Holders:© 2006 NRC Canada
Deposited On:12 Jul 2010 10:02

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