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In vitro activity of Acanthamoeba castellanii on human platelets and erythrocytes

Mattana, Antonella and Alberti, Luisa and Delogu, Giovanni and Fiori, Pier Luigi and Cappuccinelli, Pietro Antonio (2009) In vitroactivity of Acanthamoeba castellanii on human platelets and erythrocytes. Infection and Immunity, Vol. 77 (2), p. 733-738. eISSN 1098-5522. Article.

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DOI: 10.1128/IAI.00202-08

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The effect of Acanthamoeba on human platelets and erythrocytes has not been fully elucidated. This paper reports that cell-free supernatants prepared from A. castellanii can activate human platelets, causing both a significant increase in the cytosolic free-calcium concentration and platelet aggregation. In addition, we demonstrated that platelet activation depends on the activity of ADP constitutively secreted into the medium by trophozoites. This study also showed that A. castellanii can affect human red blood cells, causing hemolysis, and provided evidence that hemolysis occurs in both contact-dependent and contact-independent ways; there are differences in kinetics, hemolytic activity, and calcium dependency between the contact-dependent and contact-independent mechanisms. Partial characterization of contact-independent hemolysis indicated that ADP does not affect the plasma membrane permeability of erythrocytes and that heat treatment of amoebic cell-free supernatant abolishes its hemolytic activity. These findings suggest that some heat-labile molecules released by A. castellanii trophozoites are involved in this phenomenon. Finally, our data suggest that human platelets and erythrocytes may be potential cell targets during Acanthamoeba infection.

Item Type:Article
ID Code:4111
Uncontrolled Keywords:Acanthamoeba infection, platelets, cytosolic free-calcium, platelet aggregation, hemolysis
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 01 Dipartimenti > Scienze del farmaco
Publisher:American Society for Microbiology
Deposited On:08 Jul 2010 08:52

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