Kelsey, J. A. and Nudda, Anna and Corato, Adolfo and Mosley, Erin E. and Mosley, S. and Williams, B. K. and Grimberg, J. L. and Henderson, D. A. and Hoying, J. B. and Greer, K. and McGuire, Mark A. (2005) Assessing changes in mammary gland gene expression using a cDNA microarray in the dairy cow following administration of bovine somatotropin. Journal of Dairy Science, Vol. 88 (Suppl. 1), p. 287. eISSN 1525-3198. Article.
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To determine gene expression changes in the mammary gland following bovine somatotropin (bST) treatment, four lactating Holstein cows (253±143 DIM) were used. Mammary tissue biopsies were taken on d -5, -2, 1 and 6 relative to a single 500 mg dose of bST (Posilac®, Monsanto, St. Louis, MO). Total RNA was isolated, amplified, converted to cDNA, labeled with Cy3 or Cy5 dye, and hybridized to the NBFGC bovine microarray (Michigan State University). Comparisons were made using a modified loop design with each cow on each day represented once. Milk yield was increased (30%; P < 0.05) by d 4 and remained elevated through the end of the study. Milk fat, protein and lactose content were not affected (P > 0.1).
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