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Assessing changes in mammary gland gene expression using a cDNA microarray in the dairy cow following administration of bovine somatotropin

Kelsey, J. A. and Nudda, Anna and Corato, Adolfo and Mosley, Erin E. and Mosley, S. and Williams, B. K. and Grimberg, J. L. and Henderson, D. A. and Hoying, J. B. and Greer, K. and McGuire, Mark A. (2005) Assessing changes in mammary gland gene expression using a cDNA microarray in the dairy cow following administration of bovine somatotropin. Journal of Dairy Science, Vol. 88 (Suppl. 1), p. 287. eISSN 1525-3198. Article.

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Abstract

To determine gene expression changes in the mammary gland following bovine somatotropin (bST) treatment, four lactating Holstein cows (253±143 DIM) were used. Mammary tissue biopsies were taken on d -5, -2, 1 and 6 relative to a single 500 mg dose of bST (Posilac®, Monsanto, St. Louis, MO). Total RNA was isolated, amplified, converted to cDNA, labeled with Cy3 or Cy5 dye, and hybridized to the NBFGC bovine microarray (Michigan State University). Comparisons were made using a modified loop design with each cow on each day represented once. Milk yield was increased (30%; P < 0.05) by d 4 and remained elevated through the end of the study. Milk fat, protein and lactose content were not affected (P > 0.1).

Item Type:Article
ID Code:4089
Status:Published
Refereed:Yes
Uncontrolled Keywords:Microarray, bST, bioinformatics
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/19 Zootecnica speciale
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze zootecniche
Publisher:American Dairy Science Association
eISSN:1525-3198
Deposited On:02 Jul 2010 13:52

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