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Static cytofluorometry and fluorescence morphology of mitochondria and DNA in proliferating fibroblasts

Barni, Sergio and Sciola, Gian Luigi and Spano, Alessandra and Pippia, Proto Gavino (1996) Static cytofluorometry and fluorescence morphology of mitochondria and DNA in proliferating fibroblasts. Biotechnic and Histochemistry, Vol. 71 (2), p. 66-70. eISSN 1473-7760. Article.

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DOI: 10.3109/10520299609117135

Abstract

The shape, distribution, and content of mitochondria in individual cells were examined during the cell cycle phases (G0/G1, S, G2 mitosis) in living human fibroblasts by static cytofluorometry and fluorescence microscopy. The morphocytochemical evaluations were performed in cell cultures submitted to double supravital fluorochrome staining with Hoechst 33342 and DiOC6 to label DNA and mitochondria, respectively. The staining modalities were based on the stability of mitochondrial labeling. The G1 to early S phases were characterized by the presence of filamentous mitochondria, except during the early postmitotic period. During late S, G2, and mitotic phases, mitochondrial mass reached its highest value and mitochondria became short and numerous. During the last stage of mitosis, mitochondria were distributed among daughter cells through a cytoplasmic bridge.

Item Type:Article
ID Code:4077
Status:Published
Refereed:Yes
Uncontrolled Keywords:Static Cytofluorometry, supravital fluorochrome staining, DNA, mitochondria, cell cycle
Subjects:Area 05 - Scienze biologiche > BIO/06 Anatomia comparata e citologia
Area 05 - Scienze biologiche > BIO/09 Fisiologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Informa Healthcare
eISSN:1473-7760
Deposited On:30 Jun 2010 13:34

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