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Different apoptotic responses and patterns in adhering and floating neoplastic cell cultures: effects of microtubule antagonists

Sciola, Gian Luigi and Spano, Alessandra and Monaco, Gianni and Bottone, Maria Grazia and Barni, Sergio (2003) Different apoptotic responses and patterns in adhering and floating neoplastic cell cultures: effects of microtubule antagonists. Histochemistry and Cell Biology, Vol. 119 (1), p. 77-90. eISSN 1432-119X. Article.

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DOI: 10.1007/s00418-002-0481-4

Abstract

The relationship between apoptotic progression and cell cycle perturbation induced by microtubule-destabilising (vinblastine, Colcemid) and -stabilising (taxol) drugs was studied in two mesenchyme-derived neoplastic cell lines, growing as suspension (Jurkat) and monolayer (SGS/3A) culture, by morphocytochemical and biochemical approaches. The same kind of drug induced different effects on the cell kinetics (proliferation, polyploidisation, death) of the two cell lines. In floating cells, the drugs appeared more effective during the S phase, while in adherent cells they were more effective during the G2/M phase. Moreover two distinct neoplasia-associated apoptotic phenotypes emerged: the first pattern was the typical one and was found in cells with a low transition through the S/G2 phase (Jurkat), and the second one was mainly characterised by a cell death derived from micronucleated and mitotic cells, as a consequence of a low transition through the M/G1 phase (SGS/3A). Our data show that the machinery required for the trigger and progression of apoptosis is present in every cell cycle phase, also in conditions of karyological alterations (aneugenic micronucleations). On the other hand, a different sensitivity of the two microtubular components (interphasic network and mitotic spindle) appears to be related to the anchorage-dependence or -independence during the cell growth disturbances after exposure to antimicrotubular drugs.

Item Type:Article
ID Code:4074
Status:Published
Refereed:Yes
Uncontrolled Keywords:SGS/3A cells, Jurkat cells, apoptotic mitosis, cytoskeleton, micronuclei, microtubular drugs
Subjects:Area 05 - Scienze biologiche > BIO/06 Anatomia comparata e citologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Springer
eISSN:1432-119X
Copyright Holders:© Springer-Verlag 2002
Deposited On:30 Jun 2010 11:59

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