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Interplay between Arabidopsis activating factors E2Fb and E2Fa in cell cycle progression and development

Sozzani, Rosangela and Maggio, Caterina and Varotto, Serena and Canova, Sabrina and Bergounioux, Catherine and Albani, Diego Maria and Cella, Rino (2006) Interplay between Arabidopsis activating factors E2Fb and E2Fa in cell cycle progression and development. Plant Physiology, Vol. 140 (4), p. 1355-1366. eISSN 1532-2548. Article.

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DOI: 10.1104/pp.106.077990

Abstract

Eukaryotic E2Fs are conserved transcription factors playing crucial and antagonistic roles in several pathways related to cell division, DNA repair, and differentiation. In plants, these processes are strictly intermingled at the growing zone to produce postembryonic development in response to internal signals and environmental cues. Of the six AtE2F proteins found in Arabidopsis (Arabidopsis thaliana), only AtE2Fa and AtE2Fb have been clearly indicated as activators of E2F-responsive genes. AtE2Fa activity was shown to induce S phase and endoreduplication, whereas the function of AtE2Fb and the interrelationship between these two transcription factors was unclear. We have investigated the role played by the AtE2Fb gene during cell cycle and development performing in situ RNA hybridization, immunolocalization of the AtE2Fb protein in planta, and analysis of AtE2Fb promoter activity in transgenic plants. Overexpression of AtE2Fb in transgenic Arabidopsis plants led to striking modifications of the morphology of roots, cotyledons, and leaves that can be ascribed to stimulation of cell division. The accumulation of the AtE2Fb protein in these lines was paralleled by an increased expression of E2F-responsive G1/S and G2/M marker genes. These results suggest that AtE2Fa and AtE2Fb have specific expression patterns and play similar but distinct roles during cell cycle progression.

Item Type:Article
ID Code:4070
Status:Published
Refereed:Yes
Uncontrolled Keywords:Arabidopsis, eukaryotic E2Fs, cell cycle progression
Subjects:Area 05 - Scienze biologiche > BIO/04 Fisiologia vegetale
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze botaniche, ecologiche e geologiche
Publisher:American Society of Plant Biologists
eISSN:1532-2548
Deposited On:02 Jul 2010 13:02

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