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Mode of reproduction is detected by Parth1 and Sex1 SCAR markers in a wide range of facultative apomictic Kentucky bluegrass varieties

Albertini, Emidio and Barcaccia, Gianni and Porceddu, Andrea and Sorbolini, Silvia and Falcinelli, Mario (2001) Mode of reproduction is detected by Parth1 and Sex1 SCAR markers in a wide range of facultative apomictic Kentucky bluegrass varieties. Molecular Breeding, Vol. 7 (4), p. 293-300. eISSN 1572-9788. Article.

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DOI: 10.1023/A:1011673112747

Abstract

Gametophytic apomixis in Kentucky bluegrass (Poa pratensis L.) involves the parthenogenetic development of unreduced eggs from aposporic embryo sacs. Marker-assisted selection for the mode of reproduction in P. pratensis would avoid costly and time-consuming phenotypic progeny tests. We developed and tested two SCAR primer pairs that are associated with the mode of reproduction in P. pratensis. The SCAR primers identified the apomictic and sexual genotypes among progenies of sexual x apomictic crosses with very low bias. Furthermore, when tested on a wide range of Italian and exotic P. pratensis germplasm, they were able to unequivocally distinguish sexual from apomictic genotypes. This system should, therefore, allow new selection models to be set up in this species.

Item Type:Article
ID Code:4064
Status:Published
Refereed:Yes
Uncontrolled Keywords:AFLP, apomixis, marker-assisted selection, Poa pratensis L., SCAR
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/07 Genetica agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze zootecniche
001 Università di Sassari > 01 Dipartimenti > Scienze agronomiche e genetica vegetale agraria
Publisher:Springer Netherlands
eISSN:1572-9788
Deposited On:28 Jun 2010 17:19

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