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Transcript profiling on developing Petunia hybrida floral organs

Cnudde, Filip and Moretti, Chiaraluce and Porceddu, Andrea and Pezzotti, Mario and Gerats, Tom (2003) Transcript profiling on developing Petunia hybrida floral organs. Sexual Plant Reproduction, Vol. 16 (2), p. 77-85. eISSN 1432-2145. Article.

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DOI: 10.1007/s00497-003-0178-4

Abstract

The cDNA-AFLP transcript profiling technique was used to analyse gene expression during flower development in Petunia hybrida. Reproductive and vegetative floral organs were sampled at five developmental stages and gene expression profiles were compared. This allowed us to assemble an inventory of genes expressed mainly in anthers during microspore development and in ovaries during macrosporogenesis. About 6,000 transcript tags were generated, 354 of which showed a modulated and/or organ-specific expression pattern. Stamen-specific transcripts exhibiting an upregulation in gene expression were well represented in our screening. Ovary-specific transcripts were less frequently observed and often displayed a constant level of gene expression. Of 194 fragments characterised further by sequencing, 35% showed homology with known genes in a database search. They belong to a wide range of gene classes, such as proteases, transcription factors and genes involved in metabolism, cell cycle and disease resistance. The usefulness of cDNA-AFLP transcript profiling as a tool to unravel complex developmental processes at the molecular level is discussed.

Item Type:Article
ID Code:4062
Status:Published
Refereed:Yes
Uncontrolled Keywords:Sporogenesis, cDNA-AFLP, flower development, expression analysis, Petunia
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/07 Genetica agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze agronomiche e genetica vegetale agraria
Publisher:Springer Berlin / Heidelberg
eISSN:1432-2145
Deposited On:28 Jun 2010 17:02

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