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A Consensus list of microsatellite markers for olive genotyping

Baldoni, Luciana and Cultrera, Nicolò C. and Mariotti, Roberto and Ricciolini, Claudia and Arcioni, Sergio and Vendramin, Giovanni Giuseppe and Buonamici, Anna and Porceddu, Andrea and Sarri, Vania and Ojeda Pizarro, Maria Ángeles and Trujillo Navas, Isabel and Rallo Romero, Luis and Belaj, Angjelina and Perri, Enzo and Salimonti, Amelia and Muzzalupo, Innocenzo and Casagrande, Alberto and Lain, Orietta and Messina, Rachele and Testolin, Raffaele (2009) A Consensus list of microsatellite markers for olive genotyping. Molecular Breeding, Vol. 24 (3), p. 213-231. eISSN 1572-9788. Article.

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DOI: 10.1007/s11032-009-9285-8

Abstract

Cultivar identification is a primary concern for olive growers, breeders, and scientists. This study was aimed at examining the SSR markers retrieved from the literature and currently used in olive study, in order to select those most effective in characterizing the olive accessions and to make possible the comparison of data obtained by different laboratories. Olive microsatellite profiles were assessed by four independent laboratories, which analyzed 37 pre-selected SSR loci on a set of 21 cultivars. These SSR markers were initially tested for their reproducibility, power of discrimination and number of amplified loci/alleles. Independent segregation was tested for each pair of SSRs in a controlled cross and the allelic error rate was quantified. Some of them were finally selected as the most informative and reliable. Most of the alleles were sequenced and their sizes were determined. Profiles of the reference cultivars and a list of alleles with their sizes obtained by sequencing are reported. Several genetic parameters have been analysed on a larger set of cultivars allowing for a deeper characterization of the selected loci. Results of this study provide a list of recommended markers and protocols for olive genotyping as well as the allelic profile of a set of reference cultivars that would be useful for the establishment of a universal database of olive accessions.

Item Type:Article
ID Code:4060
Status:Published
Refereed:Yes
Uncontrolled Keywords:SSR, fingerprinting, cultivar characterization, Olea europaea, repeat motif, allelic ladder
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/07 Genetica agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze agronomiche e genetica vegetale agraria
Publisher:Springer Netherlands
eISSN:1572-9788
Deposited On:28 Jun 2010 16:39

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