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cDNA AFLP-based techniques for studying transcript profiles in horses

Cappelli, Katia and Porceddu, Andrea and Verini Supplizi, Andrea and Capomaccio, Stefano and De Marchis, Francesca and Falcinelli, Mario and Gaiti, Alberto and Silvestrelli, Maurizio (2005) cDNA AFLP-based techniques for studying transcript profiles in horses. Research in Veterinary Science, Vol. 79 (2), p. 105-112. ISSN 0034-5288. Article.

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DOI: 10.1016/j.rvsc.2004.11.006

Abstract

The identification of differentially expressed genes is a fundamental prerequisite for understanding the molecular regulation of most physiological and pathological processes. Among the procedures employed to compare mRNA populations, those that are gel-based appear to hold great promise and are considered excellent tools for studying gene expression in species, such as the equine one, for which little genomic information is available. In the present study, we evaluated two techniques for studying mRNA profiles in horse tissue, one referred to the cDNA-amplified fragment length polymorphism (AFLP) that we called C-AFLP (classical cDNA-AFLP) protocol and the other to ordered differential display (ODD) with some modifications that we named S-AFLP (systematic cDNA-AFLP). Both techniques can be applied in live animals because of the small amount of sample required. We applied the S-AFLP to investigate horse transcript profile modifications during physical exercise. We found two transcripts that are mostly expressed during exercise and immediately after the end of it.

Item Type:Article
ID Code:4057
Status:Published
Refereed:Yes
Uncontrolled Keywords:Horse, cDNA-AFLP, minimal RNA requirement, systematic sampling
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/07 Genetica agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze agronomiche e genetica vegetale agraria
Publisher:Elsevier
ISSN:0034-5288
Deposited On:28 Jun 2010 13:27

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