titoli, abstracts, parole chiave >>>
Downregulation of the Petunia hybrida α-expansin gene PhEXP1 reduces the amount of crystalline cellulose in cell walls and leads to phenotypic changes in petal limbs

Zenoni, Sara and Reale, Lara and Tornielli, Giovanni Battista and Lanfaloni, Luisa and Porceddu, Andrea and Ferrarini, Alberto and Moretti, Chiaraluce and Zamboni, Anita and Speghini, Adolfo and Ferranti, Francesco and Pezzotti, Mario (2004) Downregulation of the Petunia hybrida α-expansin gene PhEXP1 reduces the amount of crystalline cellulose in cell walls and leads to phenotypic changes in petal limbs. The Plant Cell, Vol. 16 (2), p. 295-308. eISSN 1532-298X. Article.

Full text not available from this repository.

DOI: 10.1105/tpc.018705

Abstract

The expansins comprise a family of proteins that appear to be involved in the disruption of the noncovalent bonds between cellulose microfibrils and cross-linking glycans, thereby promoting wall creep. To understand better the expansion process in Petunia hybrida (petunia) flowers, we isolated a cDNA corresponding to the PhEXP1 α-expansin gene of P. hybrida. Evaluation of the tissue specificity and temporal expression pattern demonstrated that PhEXP1 is preferentially expressed in petal limbs during development. To determine the function of PhEXP1, we used a transgenic antisense approach, which was found to cause a decrease in petal limb size, a reduction in the epidermal cell area, and alterations in cell wall morphology and composition. The diminished cell wall thickness accompanied by a reduction in crystalline cellulose indicates that the activity of PhEXP1 is associated with cellulose metabolism. Our results suggest that expansins play a role in the assembly of the cell wall by affecting either cellulose synthesis or deposition.

Item Type:Article
ID Code:4056
Status:Published
Refereed:Yes
Uncontrolled Keywords:Petunia hybrida, expansion process, PhEXP1 function, plant genetics
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/07 Genetica agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze agronomiche e genetica vegetale agraria
Publisher:American Society of Plant Biologists
eISSN:1532-298X
Deposited On:28 Jun 2010 12:59

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page