Desole, Maria Speranza and Sciola, Gian Luigi and Sircana, Sara and Godani, Chiara and Migheli, Rossana and Delogu, Maria Rosaria and Piras, Giovanna and De Natale, Guglielmo and Miele, Egidio (1998) Protective effect of deferoxamine on sodium nitroprusside-induced apoptosis in PC12 cells. Neuroscience Letters, Vol. 247 (1), p. 1-4. ISSN 0304-3940. Article.
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Reportedly, the generation of nitric oxide (NO) may lead to iron mobilization from ferritin disrupting intracellular iron homeostasis and increasing levels of reactive oxygen species. In the present study, we evaluated the role of endogenous iron in NO-induced apoptosis in PC12 cells. Apoptosis was tested by flow cytometry, fluorescence microscopy and terminal deoxynucleotidyl transferase-mediated 2′-deoxy-uridine 5′-triphosphate nick end labeling (TUNEL) technique. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. When incubated with 0.5–0.75 mM sodium nitroprusside (SNP, a chemical NO donor), PC12 cells were shown to undergo apoptosis. In addition, SNP induced a time-dependent decrease in cell viability. Since deferoxamine (0.05–0.1 mM), a powerful iron chelator, inhibited both SNP-induced apoptosis and the decrease in cell viability, we suggest that these NO effects may be dependent upon iron mobilization within the cell.
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