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Protective effect of deferoxamine on sodium nitroprusside-induced apoptosis in PC12 cells

Desole, Maria Speranza and Sciola, Gian Luigi and Sircana, Sara and Godani, Chiara and Migheli, Rossana and Delogu, Maria Rosaria and Piras, Giovanna and De Natale, Guglielmo and Miele, Egidio (1998) Protective effect of deferoxamine on sodium nitroprusside-induced apoptosis in PC12 cells. Neuroscience Letters, Vol. 247 (1), p. 1-4. ISSN 0304-3940. Article.

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DOI: 10.1016/S0304-3940(98)00260-2

Abstract

Reportedly, the generation of nitric oxide (NO) may lead to iron mobilization from ferritin disrupting intracellular iron homeostasis and increasing levels of reactive oxygen species. In the present study, we evaluated the role of endogenous iron in NO-induced apoptosis in PC12 cells. Apoptosis was tested by flow cytometry, fluorescence microscopy and terminal deoxynucleotidyl transferase-mediated 2′-deoxy-uridine 5′-triphosphate nick end labeling (TUNEL) technique. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. When incubated with 0.5–0.75 mM sodium nitroprusside (SNP, a chemical NO donor), PC12 cells were shown to undergo apoptosis. In addition, SNP induced a time-dependent decrease in cell viability. Since deferoxamine (0.05–0.1 mM), a powerful iron chelator, inhibited both SNP-induced apoptosis and the decrease in cell viability, we suggest that these NO effects may be dependent upon iron mobilization within the cell.

Item Type:Article
ID Code:4041
Status:Published
Refereed:Yes
Uncontrolled Keywords:Sodium nitroprusside, iron release, apoptosis, defaroxamine, PC12 cells
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Area 05 - Scienze biologiche > BIO/06 Anatomia comparata e citologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
Publisher:Elsevier Science Ireland
ISSN:0304-3940
Deposited On:18 Jun 2010 12:30

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