UnissResearch

Logo Universitàegli studi di Sassari
titoli, abstracts, parole chiave >>>
Relationship between actin microfilaments and plasma membrane changes during apoptosis of neoplastic cell lines in different culture conditions

Spano, Alessandra and Sciola, Gian Luigi and Monaco, Gianni and Barni, Sergio (2000) Relationship between actin microfilaments and plasma membrane changes during apoptosis of neoplastic cell lines in different culture conditions. European Journal of Histochemistry, Vol. 44 (3), p. 255-267. ISSN 1121-760X. Article.

[img]
Preview
Full text disponibile come PDF Richiede visualizzatore di PDF come GSview, Xpdf o Adobe Acrobat Reader
Available under License Creative Commons Attribution.

378Kb

Alternative URLs:

Abstract

In this study we investigated the relationship between the reorganisation of actin cytoskeleton and the changes at cell surface level (i.e. PS exposure and blebbing) in two neoplastic cell lines during apoptosis: Chang liver cells (adherent culture) and promyelocytic HL-60 cells (suspension culture), treated with the podophyllotoxin derivative VP16. The morphological analysis, performed by means of conventional fluorescence microscopy and confocal laser scanning microscopy, on Chang cells showed that onset and progress of the two processes are synchronised. The initial disassembly of stress fibers was associated with the early PS exposure on the cell surface. Moreover, the accumulation of actin at cortical level appeared strongly associated with an intense labelling for Annexin V and, in some cases, especially in the areas of membrane blebbing. The double staining for actin and PS exposure, quantitatively analysed by flow cytometry in HL-60 cells after different treatment times, demonstrated that the decrease of Annexin V binding in the late stages of apoptosis is associated with the strong reduction of actin labelling probably also due to a proteolytic cleavage. These events were also partially related to variations of 255 the functional state of mitochondria, by analysing cytofluorometrically the dissipation of the inner membrane potential (DYm).

Item Type:Article
ID Code:4039
Status:Published
Refereed:Yes
Uncontrolled Keywords:Apoptosis, actin, PS exposure, blebbing, mitochondria
Subjects:Area 05 - Scienze biologiche > BIO/06 Anatomia comparata e citologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Luigi Ponzio
ISSN:1121-760X
Copyright Holders:© Luigi Ponzio e figlio - Editori in Pavia, © 2009 PagePress
Deposited On:18 Jun 2010 10:41

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page