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Clonal diversity of Salmonella enterica serotype Typhi isolated from patients with typhoid fever in Tehran

Pourshafie, Mohammad R. and Saifi, Mahnaz and Mousavi, Seyed F. and Sedaghat, Manijeh and Nikbakht, Gholamreza H. and Rubino, Salvatore (2008) Clonal diversity of Salmonella enterica serotype Typhi isolated from patients with typhoid fever in Tehran. Scandinavian Journal of Infectious Diseases, Vol. 40 (1), p. 18-23. eISSN 1651-1980. Article.

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DOI: 10.1080/00365540701481529

Abstract

In this study, antimicrobial susceptibility test and genetic typing were used to characterize 15 Salmonella enterica serotype Typhi (S. Typhi) isolates recovered from sporadic cases of typhoid fever in Tehran, Iran during 2004. Antimicrobial susceptibility test showed that all isolates were susceptible to 20 antimicrobials examined in this study. Analysis of insertion elements showed that 2 IS200 types with 10 and 11 copies were present. 11 of the 15 isolates were found to possess 10 IS200 elements residing on fragments from 23 to 2.3 kb. Comparison of the RiboPrinter (automated ribotyping) patterns of S. Typhi showed that 60% (9/15) of the isolates belonged to a single ribotype. PCR based random amplified polymorphic DNA analysis (RAPD) and enterobacterial repetitive intergenic consensus (ERIC) and pulsed-field gel electrophresis (PFGE) were also performed. ERIC and RAPD-PCR method showed 2 and 3 genotyping patterns amongst the isolates, respectively. The PFGE typing was carried out by using XbaI restriction enzyme, and 7 restriction patterns were observed. Overall, the molecular typing methods applied in this study showed that the isolated S. Typhi populations were highly polyclonal as shown by PFGE.

Item Type:Article
ID Code:4038
Status:Published
Refereed:Yes
Uncontrolled Keywords:Salmonella, typhoid fever, Tehran
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Informa Healthcare
eISSN:1651-1980
Deposited On:18 Jun 2010 09:55

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