Porceddu, Andrea and Stals, Hilde and Reichheld, Jean-Philippe and Segers, Gerda and De Veylder, Lieven and De Pinho Barrôco, Rosa and Casteels, Peter and Montagu, Marc van and Inzé, Dirk and Mironov, Vladimir A. (2001) A Plant-specific cyclin-dependent kinase is involved in the control of G2/M progression in plants. The Journal of Biological Chemistry, Vol. 276 (39), p. 36354-36360. eISSN 1083-351X. Article.
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Cyclin-dependent kinases (CDKs) control the key transitions in the eukaryotic cell cycle. All the CDKs known to control G2/M progression in yeast and animals are distinguished by the characteristic PSTAIRE motif in their cyclin-binding domain and are closely related. Higher plants contain in addition a number of more divergent non-PSTAIRE CDKs with still obscure functions. We show that a plant-specific type of non-PSTAIRE CDKs is involved in the control of the G2/M progression. In synchronized tobacco BY-2 cells, the corresponding protein, accumulated in a cell cycle-regulated fashion, peaking at the G2/M transition. The associated histone H1 kinase activity reached a maximum in mitosis and required a yet unidentified subunit to be fully active. Down-regulation of the associated kinase activity in transgenic tobacco plants using a dominant-negative mutation delayed G2/M transition. These results provide the first evidence that non-PSTAIRE CDKs are involved in the control of the G2/M progression in plants.
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