Simoncelli, Francesca and Sorbolini, Silvia and Fagotti, Anna and Di Rosa, Ines and Porceddu, Andrea and Pascolini, Rita (2003) Molecular characterization and expression of a divergent α-tubulin in planarian Schmidtea polychroa. Biochimica et Biophysica Acta. Gene Structure and Expression, Vol. 1629 (1-3), p. 26-33. eISSN 0006-3002. Article.
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We report the cloning and sequencing of a cDNA from planarian Schmidtea polychroa (Platyhelminthes, Turbellaria, Tricladida) encoding for an unusual tubulin isoform (SpTub-1) which is specifically expressed in testis. Sequence comparison of SpTub-1 with other known tubulins reveals that it has the highest homology with α-tubulins, even though the analysis of the molecular features shows that this isoform is significantly divergent. Hybridization of SpTub-1 to restriction-digested genomic DNA to Southern blotting produced a multiple banding pattern indicating that in planarian, a tubulin multigene family exists. Using in situ hybridization, we showed that the transcript is specifically detectable in planarian testis, suggesting that it may play a role in spermatogenesis.
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