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Molecular characterization and expression of a divergent α-tubulin in planarian Schmidtea polychroa

Simoncelli, Francesca and Sorbolini, Silvia and Fagotti, Anna and Di Rosa, Ines and Porceddu, Andrea and Pascolini, Rita (2003) Molecular characterization and expression of a divergent α-tubulin in planarian Schmidtea polychroa. Biochimica et Biophysica Acta. Gene Structure and Expression, Vol. 1629 (1-3), p. 26-33. eISSN 0006-3002. Article.

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DOI: 10.1016/S0167-4781(03)00159-3

Abstract

We report the cloning and sequencing of a cDNA from planarian Schmidtea polychroa (Platyhelminthes, Turbellaria, Tricladida) encoding for an unusual tubulin isoform (SpTub-1) which is specifically expressed in testis. Sequence comparison of SpTub-1 with other known tubulins reveals that it has the highest homology with α-tubulins, even though the analysis of the molecular features shows that this isoform is significantly divergent. Hybridization of SpTub-1 to restriction-digested genomic DNA to Southern blotting produced a multiple banding pattern indicating that in planarian, a tubulin multigene family exists. Using in situ hybridization, we showed that the transcript is specifically detectable in planarian testis, suggesting that it may play a role in spermatogenesis.

Item Type:Article
ID Code:3980
Status:Published
Refereed:Yes
Uncontrolled Keywords:Tubulin gene, planarian, spermatogenesis
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/07 Genetica agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze zootecniche
001 Università di Sassari > 01 Dipartimenti > Scienze agronomiche e genetica vegetale agraria
Publisher:Elsevier
eISSN:0006-3002
Deposited On:27 May 2010 12:52

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