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Rapid detection of coinfections by Trichomonas vaginalis, Mycoplasma hominis, and Ureaplasma urealyticum by a new multiplex polymerase chain reaction

Diaz, Nicia and Dessì, Daniele and Dessole, Salvatore and Fiori, Pier Luigi and Rappelli, Paola (2010) Rapid detection of coinfections by Trichomonas vaginalis, Mycoplasma hominis, and Ureaplasma urealyticum by a new multiplex polymerase chain reaction. Diagnostic Microbiology & Infectious Disease, Vol. 67 (1), p. 30-36. ISSN 0732-8893. Article.

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DOI: 10.1016/j.diagmicrobio.2009.12.022

Abstract

We developed a multiplex polymerase chain reaction (M-PCR) assay to simultaneously detect Trichomonas vaginalis, Mycoplasma hominis, and Ureaplasma urealyticum. The test is extremely specific and has a sensitivity of 10 cells for T. vaginalis and U. Urealyticum and of 1 cell for M. hominis. The technique was validated on vaginal swabs from 240 women presenting symptoms of vaginitis, and results were compared with data obtained using microscopic and culture techniques on the same patients. The M-PCR revealed to be greatly more sensitive and specific than traditional techniques. It has been well demonstrated, in vitro, that T. vaginalis can establish a symbiosis with M. hominis; our data confirm in vivo this strict association: in fact, M. hominis has been detected in 78.6% of all samples positive for T. vaginalis, as compared to only 4.8% of women without trichomoniasis. The species specificity of this association has been confirmed by the absence of any significant correlation between T. vaginalis and U. urealyticum.

Item Type:Article
ID Code:3936
Status:Published
Refereed:Yes
Uncontrolled Keywords:Trichomonas vaginalis, Mycoplasma hominis, Ureaplasma urealyticum, multiplex PCR
Subjects:Area 06 - Scienze mediche > MED/40 Ginecologia e ostetricia
Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
Publisher:Elsevier
ISSN:0732-8893
Deposited On:25 May 2010 09:46

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