Tilocca, Maria Giovanna (2010) Applicazione di nanoparticelle magnetiche di Fe3O4 per l’isolamento ed il rilevamento di proteina prionica patologica ovina (PrPSc). Doctoral Thesis.
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Early diagnosis in Spongiform Transmissible Encephalopathies (TSE) such scrapie in
sheep and bovine spongiform encephalopathy in cow is critical in preventing the
irreversible neuronal damage that leads to fatal exitus. All approved methods of diagnosis
for TSE rely exclusively on detection of pathological prion protein (PrPSc), which is
present in the brain of the clinically affected animals.
It is well known that metal nanoparticles could serve as potential support structure for
biological material including proteins, peptides, enzymes and DNA. A range of different
nanoparticles enhance the rate of fibrillation of amyloidogenic protein and adsorb on the
surface already misfolded protein.
Here we describe that scrapie-affected sheep brain derived PrPSc is absorbed on the surface
of magnetic nanoparticles (Fe3O4) and completely removed from the aqueous solution,
where it is diluted, by using a magnetic field. PrPSc absorbed on the nanoparticles surface
was demonstrated by immunobiological assays.
These magnetic nanoparticles may, by modifying their surface with functional groups, be
useful for early in vivo detection of PrPSc in body fluids.
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