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Applicazione di nanoparticelle magnetiche di Fe3O4 per l’isolamento ed il rilevamento di proteina prionica patologica ovina (PrPSc)

Tilocca, Maria Giovanna (2010) Applicazione di nanoparticelle magnetiche di Fe3O4 per l’isolamento ed il rilevamento di proteina prionica patologica ovina (PrPSc). Doctoral Thesis.

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Abstract

Early diagnosis in Spongiform Transmissible Encephalopathies (TSE) such scrapie in sheep and bovine spongiform encephalopathy in cow is critical in preventing the irreversible neuronal damage that leads to fatal exitus. All approved methods of diagnosis for TSE rely exclusively on detection of pathological prion protein (PrPSc), which is present in the brain of the clinically affected animals.
It is well known that metal nanoparticles could serve as potential support structure for biological material including proteins, peptides, enzymes and DNA. A range of different nanoparticles enhance the rate of fibrillation of amyloidogenic protein and adsorb on the surface already misfolded protein.
Here we describe that scrapie-affected sheep brain derived PrPSc is absorbed on the surface of magnetic nanoparticles (Fe3O4) and completely removed from the aqueous solution, where it is diluted, by using a magnetic field. PrPSc absorbed on the nanoparticles surface was demonstrated by immunobiological assays.
These magnetic nanoparticles may, by modifying their surface with functional groups, be useful for early in vivo detection of PrPSc in body fluids.

Item Type:Doctoral Thesis
ID Code:3904
Publisher:Universita' degli studi di Sassari
Uncontrolled Keywords:Proteina prionica, nanoparticelle magnetiche, magnetite
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/05 Malattie infettive degli animali domestici
Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Area 07 - Scienze agrarie e veterinarie > VET/03 Patologia generale e anatomia patologica veterinaria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Cicli, scuole e corsi:Ciclo 22 > Scuole > Scienze biomolecolari e biotecnologiche > Biotecnologie molecolari e cellulari
Deposited On:18 May 2010 18:19

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