UnissResearch

Logo Universitàegli studi di Sassari
titoli, abstracts, parole chiave >>>
The cag pathogenicity island of Helicobacter pylori is disrupted in the majority of patient isolates from different human populations

Kauser, Farhana and Khan, Aleem A. and Hussain, M. Abid and Carroll, Ian M. and Ahmad, Naheed and Tiwari, Santosh and Shouche, Yogesh and Das, Bimal Kumar and Alam, Mahfooz and Ali, Shaik Mahaboob and Habibullah, Chittoor M. and Sierra, Rafaela and Megraud, Francis and Sechi, Leonardo Antonio and Ahmed, Niyaz (2004) The cag pathogenicity island of Helicobacter pylori is disrupted in the majority of patient isolates from different human populations. Journal of Clinical Microbiology, Vol. 42 (11), p. 5302-5308. eISSN 1098-660X. Article.

Full text not available from this repository.

DOI: 10.1128/JCM.42.11.5302-5308.2004

Abstract

The cag pathogenicity island (cag-PAI) is one of the major virulence determinants of Helicobacter pylori. The chromosomal integrity of this island or the lack thereof is speculated to play an important role in the progress of the gastroduodenal pathology caused by H. pylori. We determined the integrity of the cag-PAI by using specific flanking and internally anchored PCR primers to know the biogeographical distribution of strains carrying fully integral cag-PAI with proinflammatory behavior in vivo. Genotypes based on eight selected loci were studied in 335 isolates obtained from eight different geographic regions. The cag-PAI appeared to be disrupted in the majority of patient isolates throughout the world. Conservation of cag-PAI was highest in Japanese isolates (57.1%). However, only 18.6% of the Peruvian and 12% of the Indian isolates carried an intact cag-PAI. The integrity of cag-PAI in European and African strains was minimal. All 10 strains from Costa Rica had rearrangements. Overall, a majority of the strains of East Asian ancestry were found to have intact cag-PAI compared to strains of other descent. We also found that the cagE and cagT genes were less often rearranged (18%) than the cagA gene (27%). We attempted to relate cag-PAI rearrangement patterns to disease outcome. Deletion frequencies of cagA, cagE, and cagT genes were higher in benign cases than in isolates from severe ulcers and gastric cancer. Conversely, the cagA promoter and the left end of the cag-PAI were frequently rearranged or deleted in isolates linked to severe pathology. Analysis of the cag-PAI genotypes with a different biogeoclimatic history will contribute to our understanding of the pathogen-host interaction in health and disease.

Item Type:Article
ID Code:3887
Status:Published
Refereed:Yes
Uncontrolled Keywords:Helicobacter pylori, cag pathogenicity island, cag-PAI genotypes
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:American Society for Microbiology
eISSN:1098-660X
Deposited On:17 May 2010 13:27

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page