Mura, Antonia (2010) Studio dell'espressione di una Flavon Diiron Protein (FDP) in Giardia intestinalis. Doctoral Thesis.
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Giardia lamblia is a flagellated unicellular eukaryotic microorganism that
commonly causes diarrheal disease throughout the world. Although Giardia has a
poor tolerance to O2, it preferentially colonizes the fairly aerobic upper part of the
The flavodiiron proteins (FDP) are widespread among strict or facultative
anaerobic prokaryotes, where they are involved in the response to nitrosative
and/or oxidative stress. The FDP from Giardia has high O2-reductase activity but
very low NO-reductase activity; O2 reacts with the reduced protein quite rapidly
and with high affinity producing H2O.
Experiments were designed to examine whether the FDP recombinant, stimulate
the immune system of the host (mouse) producing specific antibodies. To verify if
they specifically recognize the native protein in Giardia, the antibodies have been
used to set up by blotting and fluorescence techniques.
Experiments were performed under conditions of stress in order to obtain
information on the location and expression of the native protein.
I wanted to study the overexpression of the protein to determine its intracellular
location and confirm the data obtained with not transformed cells.
Further characterization experiments have led to study the protein expression
during encystations and to evaluating the expression of FDP in metronidazoleresistant
cells compared to drug sensitive protozoa.
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