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Air-liquid biofilm formation is independent on RAS2 gene in Saccharomyces cerevisiae flor yeasts

Zara, Severino and Zara, Giacomo and Farris, Giovanni Antonio and Mannazzu, Ilaria Maria and Budroni, Marilena (2009) Air-liquid biofilm formation is independent on RAS2 gene in Saccharomyces cerevisiae flor yeasts. Annals of Microbiology, Vol. 59 (Special Issue), p. 85. ISSN 1590-4261. Article.

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DOI: 10.1007/BF03178830

Abstract

In Saccharomyces cerevisiae RAS2 gene switches on cAMP-PKA and MAPK pathways (Sengupta et al., 2006) that, in turn, regulate FLO11 gene. This gene is involved in many cell-cell adhesion phenotypes like pseudohyphae formation, adhesive growth and biofilm formation (Reynolds and Fink, 2001; Zara et al., 2005; Fidalgo et al., 2006). The aim of this work was to assess the involvement of RAS2 gene in the airliquid biofilm formation by Saccharomyces cerevisiae flor strains. To do that RAS2 gene was deleted in the Saccharomyces cerevisiae flor strain 3238-32 and both the wild type and the ras2D mutant were tested for biofilm formation and FLO11 transcription levels. ras2D maintained the ability to form biofilm, while significant differences were observed in FLO11 transcriptional levels. Finally, the absence of glucose and the presence of nitrogen enhanced air-liquid biofilm formation both in the parental and ras2D flor strains. Thus, RAS2 deletion does not affect air-liquid biofilm formation.

Item Type:Article
ID Code:3831
Status:Published
Refereed:Yes
Uncontrolled Keywords:Flor, Saccharomyces cerevisiae, RAS2, biofilm
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/16 Microbiologia agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze ambientali agrarie e biotecnologie agro-alimentari
Publisher:University of Milan, Department of Food Science and Microbiology
ISSN:1590-4261
Additional Information:Poster presented at The role of Microbiology in Agricultural, Food and Environmental Fields: the 2nd National Conference SIMTREA, Sassari, Italy, June 10-12, 2009.
Deposited On:06 May 2010 08:46

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