Zara, Severino and Zara, Giacomo and Farris, Giovanni Antonio and Mannazzu, Ilaria Maria and Budroni, Marilena (2009) Air-liquid biofilm formation is independent on RAS2 gene in Saccharomyces cerevisiae flor yeasts. Annals of Microbiology, Vol. 59 (Special Issue), p. 85. ISSN 1590-4261. Article.
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In Saccharomyces cerevisiae RAS2 gene switches on cAMP-PKA and MAPK pathways (Sengupta et al., 2006) that, in turn, regulate FLO11 gene. This gene is involved in many cell-cell adhesion phenotypes like pseudohyphae formation, adhesive growth and biofilm formation (Reynolds and Fink, 2001; Zara et al., 2005; Fidalgo et al., 2006). The aim of this work was to assess the involvement of RAS2 gene in the airliquid biofilm formation by Saccharomyces cerevisiae flor strains. To do that RAS2 gene was deleted in the Saccharomyces cerevisiae flor strain 3238-32 and both the wild type and the ras2D mutant were tested for biofilm formation and FLO11 transcription levels. ras2D maintained the ability to form biofilm, while significant differences were observed in FLO11 transcriptional levels. Finally, the absence of glucose and the presence of nitrogen enhanced air-liquid biofilm formation both in the parental and ras2D flor strains. Thus, RAS2 deletion does not affect air-liquid biofilm formation.
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