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SED1 polymorphism within the genus Saccharomyces

Marinangeli, Paola and Clementi, Francesca and Ciani, Maurizio and Mannazzu, Ilaria Maria (2004) SED1polymorphism within the genus Saccharomyces. FEMS Yeast Research, Vol. 5 (1), p. 73-79. eISSN 1567-1356. Article.

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DOI: 10.1016/j.femsyr.2004.07.001

Abstract

The SED1 gene is characterised by abundant length and sequence polymorphisms within the species Saccharomyces cerevisiae, due to the expansion and contraction of minisatellite-like sequences located within the ORF. A survey of the SED1 ORFs of 26 yeasts ascribed to the species S. cerevisiae, S. bayanus, S. pastorianus, S. paradoxus, S. cariocanus, S. kudriavzevii and S. mikatae revealed SED1 gene length and sequence variations between the species of the genus. Moreover, results obtained by Neighbour-Joining analysis of a dataset comprising the partial predicted amino acid sequences of SED1 ORFs agreed with the phylogenetic relationships of the seven species. Thus, the SED1 gene may represent a further molecular target for the identification of Saccharomyces isolates.

Item Type:Article
ID Code:3819
Status:Published
Refereed:Yes
Uncontrolled Keywords:SED1, Saccharomyces, gene length polymorphism, PCR-RFLP, yeast identification
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/16 Microbiologia agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze ambientali agrarie e biotecnologie agro-alimentari
Publisher:Elsevier
eISSN:1567-1356
Copyright Holders:© 2004 Federation of European Microbiological Societies
Deposited On:04 May 2010 10:06

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