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Amplified ribosomal DNA restriction analysis for the characterization of Azotobacteraceae: a contribution to the study of these free-living nitrogen-fixing bacteria

Aquilanti, Lucia and Mannazzu, Ilaria Maria and Papa, Roberto and Cavalca, Lucia and Clementi, Francesca (2004) Amplified ribosomal DNA restriction analysis for the characterization of Azotobacteraceae: a contribution to the study of these free-living nitrogen-fixing bacteria. Journal of Microbiological Methods, Vol. 57 (2), p. 197-206. ISSN 0167-7012. Article.

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DOI: 10.1016/j.mimet.2004.01.006

Abstract

A 16S rRNA gene-based fingerprinting method was developed for the identification of Azotobacteraceae and tested onto 48 soil isolates and 28 reference strains belonging to the free-living nitrogen-fixing bacterial group and to the most common species found in soil samples. According to this method, the 16S rRNA gene was amplified using universal primers for Eubacteria and PCR products were subsequently digested with RsaI, HhaI, HpaII, FnuDII, and AluI. The analysis of the restriction profiles obtained showed that the method is able to define a unique species-specific phylotype (SSP) for each of the eight Azotobacteraceae species tested. Cluster analysis was successfully employed for the identification of members of the family Azotobacteraceae, being assignation into species of the isolates confirmed by means of partial 16S rRNA gene sequencing.

Item Type:Article
ID Code:3813
Status:Published
Refereed:Yes
Uncontrolled Keywords:Azotobacter, Azotobacteraceae, ARDRA, cluster analysis
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/16 Microbiologia agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze ambientali agrarie e biotecnologie agro-alimentari
Publisher:Elsevier
ISSN:0167-7012
Deposited On:03 May 2010 18:17

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