Mattana, Antonella and Bennardini, Federico and Juliano, Claudia Clelia Assunta and Picci, V. and Marceddu, Salvatore and Sciola, Gian Luigi and Pippia, Proto Gavino and Franconi, Flavia (1994) Cytotoxicity of lazaroid U-75412E in human epithelial cell line (Wish). Biochemical Pharmacology, Vol. 48 (2). ISSN 0006-2952. Article.
Full text not available from this repository.
The 21-aminosteroids (or lazaroids) are a recently synthesized class of compounds demonstrated to protect tissue against damage induced by trauma and/or ischemia. Currently, very little is known about the biological effects of lazaroids. In this work the action of lazaroid U-75412E on a human epithelial cell line (Wish) was evaluated. The data obtained showed an inhibition of cell growth and a dose- and time-dependent decrease of cell viability. Furthermore, a dose- and timedependent increase of cells in the G2/M phase with the appearence of apoptotic cells was observed by flow cytometric analysis. Nuclear fragmentation was also evident. Lactate dehydrogenase release and scanning electron microscopy experiments suggested that plasma membrane integrity was altered by this compound. The immunofluorescence technique and transmission electron microscopy images also showed intracellular damage, such as alteration of microtubular arrangement, mitochondrial swelling and the presence of vacuoles. This study demonstrated that 1 μM U-75412E was unable to modify these parameters, while higher concentrations (6–75 μM) had a cytotoxic effect on Wish cells.
I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore
Repository Staff Only: item control page