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Cytotoxicity of lazaroid U-75412E in human epithelial cell line (Wish)

Mattana, Antonella and Bennardini, Federico and Juliano, Claudia Clelia Assunta and Picci, V. and Marceddu, Salvatore and Sciola, Gian Luigi and Pippia, Proto Gavino and Franconi, Flavia (1994) Cytotoxicity of lazaroid U-75412E in human epithelial cell line (Wish). Biochemical Pharmacology, Vol. 48 (2). ISSN 0006-2952. Article.

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DOI: 10.1016/0006-2952(94)90096-5

Abstract

The 21-aminosteroids (or lazaroids) are a recently synthesized class of compounds demonstrated to protect tissue against damage induced by trauma and/or ischemia. Currently, very little is known about the biological effects of lazaroids. In this work the action of lazaroid U-75412E on a human epithelial cell line (Wish) was evaluated. The data obtained showed an inhibition of cell growth and a dose- and time-dependent decrease of cell viability. Furthermore, a dose- and timedependent increase of cells in the G2/M phase with the appearence of apoptotic cells was observed by flow cytometric analysis. Nuclear fragmentation was also evident. Lactate dehydrogenase release and scanning electron microscopy experiments suggested that plasma membrane integrity was altered by this compound. The immunofluorescence technique and transmission electron microscopy images also showed intracellular damage, such as alteration of microtubular arrangement, mitochondrial swelling and the presence of vacuoles. This study demonstrated that 1 μM U-75412E was unable to modify these parameters, while higher concentrations (6–75 μM) had a cytotoxic effect on Wish cells.

Item Type:Article
ID Code:381
Status:Published
Refereed:Yes
Uncontrolled Keywords:Cytotoxicity, lazaroids, wish cells, apoptosis
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Area 03 - Scienze chimiche > CHIM/09 Farmaceutico tecnologico applicativo
Area 05 - Scienze biologiche > BIO/06 Anatomia comparata e citologia
Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Area 05 - Scienze biologiche > BIO/09 Fisiologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto di arboricoltura mediterranea, Sassari
001 Università di Sassari > 01 Dipartimenti > Scienze del farmaco
Publisher:Elsevier
ISSN:0006-2952
Deposited On:18 Aug 2009 10:02

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