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Metodologie per la quantificazione in campo dell'azotofissazione simbiotica delle leguminose

Roggero, Pier Paolo and Ballatore, Benedetto Francesco and Sulas, Leonardo (2003) Metodologie per la quantificazione in campo dell'azotofissazione simbiotica delle leguminose. Rivista di agronomia, Vol. 37 (2), p. 207-226. ISSN 0035-6034. Article.

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Abstract

This paper has been written in the context of the Italian national research project (PRIN) "Physiology of production and nitrogen fixation of forage legumes in eco-compatible farming systems", with the aim of analysing the state of the art on the main methods to estimate symbiotic nitrogen fixation of legumes in the field. Assumptions, procedures, advantages and limits of the following methods are illustrated: nitrogen balance (NB), acetylene reduction assay (ARA), 15N2 isotope reduction (IR), 15N isotopic dilution (ID), 15N natural abundance (NA) and xylem sap analysis. Some applications on the estimate of below-ground nitrogen of legumes and the implications on rotations and mixtures are also described.
The developrnent of methods based on the nitrogen isotopic discrimination, particularly ID and NA methods, have substantially contributed to overcome the limitations of old methods such as ARA and NB. However, new methods can be misused if fundamental assumptions are not satisfied, such as the time-space uniformity of soil 15N enrichment and the similar N uptake dynamics of the fixing and reference species. Modelling approaches can help to mitigate the assumption violations.
It is not available a standard methodology to assess the below-ground fixed mtrogen of any legume species, as specific procedures should be developed for each species and experimental site. As this is a relevant point from an agronomic perspective, further research is needed to assess below-ground nitrogen of legumes and the amount of symbiotic fixed nitrogen which enters the nitrogen cycle. Particular attention should also be deserved to the development of adequate methods to exploit the results of the field experiments involving main stakeholders of the legume-based production systems.

Item Type:Article
ID Code:3757
Status:Published
Refereed:Yes
Uncontrolled Keywords:Symbiotic N2-fixation, nitrogen balance, 15N isotope reduction, 15N isotope dilution, natural abundance, xylem sap analysis, below-ground nitrogen, cropping systems
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/02 Agronomia e coltivazioni erbacee
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze agronomiche e genetica vegetale agraria
002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto per il sistema produzione animale in ambiente Mediterraneo, Sassari
Publisher:Forum editrice udinese
ISSN:0035-6034
Copyright Holders:© 2003 Forum Editrice Universitaria Udinese
Publisher Policy:Depositato per gentile concessione dell'Editore e della Società italiana di agronomia
Deposited On:22 Apr 2010 08:54

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