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Plasma methionine determination by capillary electrophoresis–UV assay: application on patients affected by retinal venous occlusive disease

Zinellu, Angelo and Sotgia, Salvatore and Usai, Maria Franca and Zinellu, Elisabetta and Posadino, Anna Maria and Gaspa, Leonardo and Chessa, Roberto and Pinna, Antonio <1963- > and Carta, Francesco and Deiana, Luca and Carru, Ciriaco (2007) Plasma methionine determination by capillary electrophoresis–UV assay: application on patients affected by retinal venous occlusive disease. Analytical Biochemistry, Vol. 363 (1), p. 91-96. ISSN 0003-2697. Article.

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DOI: 10.1016/j.ab.2007.01.009

Abstract

Methionine is an important amino acid involved in protein synthesis and transmethylation reactions. It is also the precursor of homocysteine and cysteine, two important risk factors for cardiovascular diseases. As homocysteine research has gained impulsion, the evaluation of plasma methionine concentrations has acquired importance. Methionine measurement generally has been performed by HPLC after o-phthalaldehyde derivatization. Its separation from other amino acids is time-consuming. We set up a new specific capillary electrophoresis method in which analyte derivatization was avoided by sample concentration before analysis. Methionine was detected by UV absorbance at 204 nm with a detection limit of 0.5 μmol/L. By a capillary with an effective length of 50 cm filled with 125 mmol/L Tris phosphate buffer at pH 2.3, the separation occurred in less than 14 min. Precision tests indicated a good test repeatability for both migration times (coefficient of variation [CV] < 0.3%) and areas (CV < 2.0%). Moreover, a good reproducibility of intraassay and interassay tests was obtained (CV < 2.9% and CV < 3.5%, respectively). The Passing–Bablok regression and the Bland–Altman test for methods comparison suggest that the data obtained by our method and by a reference HPLC assay are similar. Assay performance was evaluated measuring methionine concentrations in retinal venous occlusive disease.

Item Type:Article
ID Code:3702
Status:Published
Refereed:Yes
Uncontrolled Keywords:Methionine, capillary electrophoresis, atherosclerosis, RVO
Subjects:Area 06 - Scienze mediche > MED/30 Malattie apparato visivo
Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 03 Istituti > Oftalmologia, Otorinolaringoiatria e Urologia
002 Altri enti e centri di ricerca del Nord Sardegna > National laboratory of the national institute of biostructures and biosystems, Osilo
Publisher:Academic Press inc. Elsevier Science
ISSN:0003-2697
Copyright Holders:© 2007 Elsevier
Deposited On:09 Apr 2010 12:03

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