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Exposure of human lymphocytes and lymphoblastoid cells to simulated microgravity strongly affects energy metabolism and DNA repair

Degan, Paolo and Sancandi, Monica and Zunino, Annalisa and Ottaggio, Laura and Viaggi, Silvia and Cesarone, Carmelo Federico and Pippia, Proto Gavino and Galleri, Grazia and Abbondandolo, Angelo (2005) Exposure of human lymphocytes and lymphoblastoid cells to simulated microgravity strongly affects energy metabolism and DNA repair. Journal of Cellular Biochemistry, Vol. 94 (3), p. 460-469. eISSN 1097-4644. Article.

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DOI: 10.1002/jcb.20302

Abstract

Exposure of freshly drawn lymphocytes and lymphoblastoid cells (LB and COR3) to simulated microgravity decreased the intracellular ATP concentration to 50%-40% of the value found in normal growth conditions. The decrease was reversible although recovery to normal values occurred only slowly both in lymphocytes and in lymphoblastoid cells. Poly(ADP-ribose) polymerase (PARP ) activity was increased indicating that cells exposed to conditions of reduced gravitation experience stress. Exposure to microgravity forces cells into a condition of metabolic quiescence in which they appear to be particularly sensitive to subsequent exposures to a genotoxic agent. Thus, treatment of cells with the strong redox agent potassium bromate under microgravity conditions, indicated an impairment in repair of DNA 8-hydroxy-2'-deoxyguanosine (8-OHdG), an oxidized derivative of deoxyguanosine. We conclude that gravitational modulation of the kind routinely obtained under laboratory conditions and during spaceflights is a stressful process to which cells appear to be extremely sensitive. These effects may reflect the physiological alterations observed in astronauts and in animals following spaceflights or exposure to conditions of simulated microgravity.

Item Type:Article
ID Code:370
Status:Published
Refereed:Yes
Uncontrolled Keywords:Microgravity, energy metabolism, DNA repair, 8-hydroxy-2-deoxyguanosine, apoptosis
Subjects:Area 05 - Scienze biologiche > BIO/09 Fisiologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Wiley-Liss
eISSN:1097-4644
Deposited On:18 Aug 2009 10:02

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