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An RP-HPLC determination of 5-hydroxymethylfurfural in honey: the case of strawberry tree honey

Spano, Nadia and Casula, Lucia and Panzanelli, Angelo and Pilo, Maria Itria and Piu, Paola Costantina and Scanu, Roberta and Tapparo, Andrea and Sanna, Gavino (2006) An RP-HPLC determination of 5-hydroxymethylfurfural in honey: the case of strawberry tree honey. Talanta, Vol. 68 (4), p. 1390-1395. ISSN 0039-9140. Article.

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DOI: 10.1016/j.talanta.2005.08.003


The use of the RP-HPLC official method of the International Honey Commission (IHC) for the determination of 5-hydroxymethylfurfural (HMF) in strawberry tree honey (Arbutus unedo, a typical Sardinian honey) has brought to light a specific and heavy chromatographic interference that prevents accurate quantification. The interference has been identified as homogentisic acid (HA), i.e. the marker of the botanical origin of the honey. For this reason, an alternative RP-HPLC method is proposed. The bias-free method allows a complete separation of HMF from HA to the baseline level and is faster and more precise than the RP-HPLC official method: the detection and quantification limits are 1.9 and 4.0 mg kg-1, respectively, whereas the repeatability is ca. 2% in the HMF concentration range of 5-140 mg kg-1.

Item Type:Article
ID Code:3689
Uncontrolled Keywords:Strawberry tree honey, 5-hydroxymethylfurfural, homogentisic acid, RP-HPLC
Subjects:Area 03 - Scienze chimiche > CHIM/01 Chimica analitica
Divisions:001 Università di Sassari > 01 Dipartimenti > Chimica
Deposited On:06 Apr 2010 13:57

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