Piga, Carlo and Urgeghe, Pietro Paolo and Piredda, Giovanni and Scintu, Maria Francesca and Sanna, Gavino (2009) Assessment and validation of methods for the determination of γ-glutamyltransferase activity in sheep milk. Food Chemistry, Vol. 115 (4), p. 1519-1523. ISSN 0308-8146. Article.
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The determination of enzymatic activity in sheep milk is still today a practically unexplored field of research. This study proposes and fully validates two analytical procedures (i.e. by means of UV-vis spectrophotometry and RP-HPLC methods) for the determination of γ-glutamyltransferase activity in sheep milk. Both methods are characterised by low detection and quantification limits, excellent linearity over a wide enzymatic activity interval, very good repeatability and reproducibility, and are bias-free. The RP-HPLC method provides better sensitivity and automation capability levels than that of UV-vis, and its use is hence suggested for screening purposes. These methods have been preliminarily tested with a number of real samples of whole sheep milk, obtaining an average γ-glutamyltransferase activity value of 2.93 ± 0.50 U ml−1 and a range from 2.72 ± 0.10 U ml−1 to 3.46 ± 0.11 U ml−1.
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