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Assessment and validation of methods for the determination of γ-glutamyltransferase activity in sheep milk

Piga, Carlo and Urgeghe, Pietro Paolo and Piredda, Giovanni and Scintu, Maria Francesca and Sanna, Gavino (2009) Assessment and validation of methods for the determination of γ-glutamyltransferase activity in sheep milk. Food Chemistry, Vol. 115 (4), p. 1519-1523. ISSN 0308-8146. Article.

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DOI: 10.1016/j.foodchem.2009.01.041

Abstract

The determination of enzymatic activity in sheep milk is still today a practically unexplored field of research. This study proposes and fully validates two analytical procedures (i.e. by means of UV-vis spectrophotometry and RP-HPLC methods) for the determination of γ-glutamyltransferase activity in sheep milk. Both methods are characterised by low detection and quantification limits, excellent linearity over a wide enzymatic activity interval, very good repeatability and reproducibility, and are bias-free. The RP-HPLC method provides better sensitivity and automation capability levels than that of UV-vis, and its use is hence suggested for screening purposes. These methods have been preliminarily tested with a number of real samples of whole sheep milk, obtaining an average γ-glutamyltransferase activity value of 2.93 ± 0.50 U ml−1 and a range from 2.72 ± 0.10 U ml−1 to 3.46 ± 0.11 U ml−1.

Item Type:Article
ID Code:3688
Status:Published
Refereed:Yes
Uncontrolled Keywords:γ-Glutamyltransferase, enzymatic activity, sheep milk, spectrophotometry, RP-HPLC
Subjects:Area 03 - Scienze chimiche > CHIM/01 Chimica analitica
Area 07 - Scienze agrarie e veterinarie > AGR/15 Scienze e tecnologie alimentari
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > AGRIS Sardegna, Olmedo > Dipartimento per la ricerca nelle produzioni animali
001 Università di Sassari > 01 Dipartimenti > Chimica
001 Università di Sassari > 01 Dipartimenti > Scienze ambientali agrarie e biotecnologie agro-alimentari
Publisher:Elsevier
ISSN:0308-8146
Deposited On:06 Apr 2010 13:23

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