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Analytical methods for the evaluation of α-L-fucosidase activity in sheep milk

Piga, Carlo and Urgeghe, Pietro Paolo and Piredda, Giovanni and Scintu, Maria Francesca and Sanna, Gavino (2010) Analytical methods for the evaluation of α-L-fucosidase activity in sheep milk. Food Analytical Methods, Vol. 3 (1), p. 17-21. eISSN 1936-976X. Article.

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DOI: 10.1007/s12161-008-9069-5

Abstract

The lack of analytical methods for measuring the activity of highly thermolable endogenous enzymes in sheep milk is a factor that hampers the protection of typical Protected Designation of Origin (PDO) dairy products made from raw milk. In order to provide a solution, this study assesses, tests, and fully validates analytical procedures for the determination of α-L-fucosidase activity in sheep milk. While the UV–VIS method has been optimized for this matrix in order to solve clarification problems before the spectrophotometric reading, a reversed phase high-performance liquid chromatography literature method proposed for bovine milk has been successfully applied also to sheep milk. Both methods have been fully validated in terms of sensitivity, linearity, precision, and accuracy, displaying low detection and quantification limits, excellent linearity over a wide enzymatic activity interval, very good repeatability and reproducibility, and the lack of any bias. The analysis of a number of real samples of whole sheep milk has allowed the evaluation of an average value (46.78 ± 5.49 U mL−1) and range (from 29.27 ± 2.60 to 72.64 ± 1.17 U mL−1) of α-L-fucosidase activity. Such activity does not seem to differ substantially from those measured for bovine milk. Finally, marked seasonal variability has been observed in this preliminary dataset.

Item Type:Article
ID Code:3675
Status:Published
Refereed:Yes
Uncontrolled Keywords:α-l-fucosidase, enzymatic activity, sheep milk, spectrophotometry, RP-HPLC
Subjects:Area 03 - Scienze chimiche > CHIM/01 Chimica analitica
Area 07 - Scienze agrarie e veterinarie > AGR/15 Scienze e tecnologie alimentari
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > AGRIS Sardegna, Olmedo > Dipartimento per la ricerca nelle produzioni animali
001 Università di Sassari > 01 Dipartimenti > Chimica
001 Università di Sassari > 01 Dipartimenti > Scienze ambientali agrarie e biotecnologie agro-alimentari
Publisher:Springer New York
eISSN:1936-976X
Deposited On:01 Apr 2010 13:58

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