UnissResearch

Logo Universitàegli studi di Sassari
titoli, abstracts, parole chiave >>>
Reversed-Phase liquid chromatographic profile of free amino acids in strawberry-tree (Arbutus unedo L.) honey

Spano, Nadia and Piras, Irene and Ciulu, Marco and Floris, Ignazio and Panzanelli, Angelo and Pilo, Maria Itria and Piu, Paola Costantina and Sanna, Gavino (2009) Reversed-Phase liquid chromatographic profile of free amino acids in strawberry-tree (Arbutus unedo L.) honey. Journal of AOAC International, Vol. 92 (4), p. S1145-S1156. ISSN 1060-3271. Article.

Full text not available from this repository.

Alternative URLs:

Abstract

The typical profile of the free amino acids contained in strawberry-tree (Arbutus unedo L.) honey is reported for the first time. An optimized reversed-phase liquid chromatographic (RP-LC) method with phenyl isothiocyanate precolumn derivatization was used. Fourteen free amino acids were identified and quantified in 16 analytical samples. Proline (65.63%) was found to be the most abundant free amino acid, followed by glutamic acid (6.49%), arginine (5.21%), alanine (5.17%), and phenylalanine (4.97%). The total free amino acid content of strawberry-tree honey (average value, 436 mg/kg) was found to be low in comparison to amounts cited in the literature concerning unifloral honeys. The analytical method was optimized and fully validated in terms of detection and quantitation limits, precision (by testing repeatability and reproducibility), linearity, and bias (by means of recovery tests). The acceptability of the validation protocol results was verified using Horwitz's mathematical model and AOAC guidelines.

Item Type:Article
ID Code:3674
Status:Published
Refereed:Yes
Uncontrolled Keywords:Arbutus unedo L., free amino acids, strawberry-tree honey
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/11 Entomologia generale e applicata
Area 03 - Scienze chimiche > CHIM/01 Chimica analitica
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
001 Università di Sassari > 01 Dipartimenti > Chimica
Publisher:AOAC International
ISSN:1060-3271
Deposited On:01 Apr 2010 13:27

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page