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Studies of interaction between Rh(I) and human serum albumin in a “nanostructured biocatalyst” active in the hydroformylation reaction

Crobu, Salvatore and Marchetti, Mauro and Sanna, Gavino (2006) Studies of interaction between Rh(I) and human serum albumin in a “nanostructured biocatalyst” active in the hydroformylation reaction. Journal of Inorganic Biochemistry, Vol. 100 (9), p. 1514-1520. ISSN 0162-0134. Article.

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DOI: 10.1016/j.jinorgbio.2006.04.014

Abstract

The interaction between Rh(CO)2(acac = acetylacetonate ion) and human serum albumin (HSA) in a hydrosoluble nanostructured biocatalyst active in homogeneous hydroformylation was characterised by means of matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS), high performance liquid chromatography mass spectrometry (HPLC-MS) and scanning electron microscopy (SEM). MALDI-TOFMS substantiates that the biocatalyst consists of a tetrameric structure of HSA that could bind up to 89 Rh(CO)+2 units. A comparison between samples of pure HSA and the biocatalyst, both tryptic digested, showed a significant change in the tertiary structure of the protein in the HSA/Rh adduct, probably ascribable to the interaction of Rh(I) ions with sulphur atoms in the HSA moiety. SEM observations confirmed an evident denaturation of the protein and an outstanding correspondence between the surface distribution of Rh and S atoms; this is indirect evidence that the metal ion interacts strongly mainly with the sulphur atoms. Furthermore, an excellent agreement between calculated and measured (SEM) S/Rh elemental mean ratio was observed. Finally, an electrostatic interaction between Rh(I) and sulphur atoms was ruled out by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) findings.

Item Type:Article
ID Code:3672
Status:Published
Refereed:Yes
Uncontrolled Keywords:Rh(I), human serum albumin, nanostructured biocatalyst, MALDI-TOFMS
Subjects:Area 03 - Scienze chimiche > CHIM/01 Chimica analitica
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Porto Conte Ricerche, Alghero
001 Università di Sassari > 01 Dipartimenti > Chimica
001 Università di Sassari > 02 Centri > Nanoworld Institute, Nanoscience Laboratories, Sassari Unit
002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto di chimica biomolecolare, Sassari
Publisher:Elsevier Science
ISSN:0162-0134
Deposited On:01 Apr 2010 12:34

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