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Genetic characterization of Fasciola hepatica from Tunisia and Algeria based on mitochondrial and nuclear DNA sequences

Farjallah, Sara and Sanna, Daria and Amor, Nabil and Ben Mehel, Benakriche and Piras, Maria Cristina and Merella, Paolo and Casu, Marco and Curini Galletti, Marco and Said, Khaled and Garippa, Giovanni (2009) Genetic characterization of Fasciola hepatica from Tunisia and Algeria based on mitochondrial and nuclear DNA sequences. Parasitology Research, Vol. 105 (6), p. 1617-1621. eISSN 1432-1955. Article.

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DOI: 10.1007/s00436-009-1601-z

Abstract

Fasciolosis caused by Fasciola spp. (Platyhelminthes: Trematoda: Digenea) is considered the most important helminth infection of ruminants in tropical countries, causing considerable socioeconomic problems. In the present study, samples identified morphologically as Fasciola hepatica from sheep and cattle from different geographical locations of Tunisia and Algeria were genetically characterised by sequences of the first (ITS-1), the 5.8S and second (ITS-2) Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA) and mitochondrial Cytochrome c Oxidase subunit I (COI) gene. Comparison of the ITS and COI sequences of the North African samples with sequences of Fasciola spp. from GenBank confirmed that all samples from Tunisia and Algeria samples belong to a single species, namely F. hepatica. Several specimens from Tunisia and Algeria showed a substitution C/T in position 859 in the ITS-2 sequences, previously reported from Spain, suggesting that the above mentioned variant may have a common origin and spread recently throughout the three countries because of movement of infected animals. This is the first molecular characterization of F. hepatica in North Africa which provides a foundation for further studies on Fasciola spp. in Tunisia and Algeria.

Item Type:Article
ID Code:3644
Status:Published
Refereed:Yes
Uncontrolled Keywords:Fasciola spp., helminth infection, ruminants
Subjects:Area 05 - Scienze biologiche > BIO/05 Zoologia
Area 07 - Scienze agrarie e veterinarie > VET/06 Parassitologia e malattie parassitarie degli animali
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
001 Università di Sassari > 01 Dipartimenti > Zoologia e genetica evoluzionistica
Publisher:Springer Berlin / Heidelberg
eISSN:1432-1955
Deposited On:26 Mar 2010 22:49

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