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Molecular phylogenetic diversity of dermatologic and other human pathogenic Fusaria isolates from hospitals in Northern and Central Italy

Migheli, Quirico and Balmas, Virgilio and Harak, Henry and Sanna, Silvana and Scherm, Barbara and Aoki, Takayuki and O'Donnell, Kerry (2010) Molecular phylogenetic diversity of dermatologic and other human pathogenic Fusaria isolates from hospitals in Northern and Central Italy. Journal of Clinical Microbiology, Vol. 48 (4), p. 1076-1084. eISSN 1098-660X. Article.

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DOI: 10.1128/JCM.01765-09

Abstract

Fifty-eight fusaria isolated from 50 Italian patients between 2004 and 2007 were subject to multilocus DNA sequence typing to characterize the spectrum of species and circulating sequence types (STs) associated with dermatological infections, especially onychomycoses and paronychia, and other fusarioses in Northern and Central Italy. Sequence typing revealed that the isolates were nearly evenly divided among the Fusarium solani (FSSC, N =18), the F. oxysporum (FOSC, N = 20) and Gibberella (Fusarium) fujikuroi (GFSC, N = 20) species complexes. The three-locus typing scheme used for members of the FSSC identified 18 novel STs distributed among six phylogenetically distinct species, yielding an index of discrimination of 1.0. Phylogenetic analysis of the FOSC two-locus dataset identified nine STs, including four which were novel, and nine isolates of ST 33, the previously described widespread clonal lineage. With the inclusion of eight epidemiologically unrelated ST 33 isolates, the FOSC typing scheme scored a discrimination index of 0.787. The two-locus GFSC typing scheme, which was designed primarily to identify species, received the lowest discrimination index with a score of 0.492. The GFSC scheme, however, was used to successfully identify 17 isolates as F. verticillioides, two as F. sacchari and one as F. guttiforme. The present study represents the first report of the latter species causing a human mycotic infection, which was supported by detailed morphological analysis. In addition, results of a pathogenicity experiment revealed that the human isolate of F. guttiforme was able to induce fusariosis of pineapple, heretofore its only known host.

Item Type:Article
ID Code:3600
Status:Published
Refereed:Yes
Uncontrolled Keywords:F. guttiforme, DNA sequence, mycotic infection
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
002 Altri enti e centri di ricerca del Nord Sardegna > Istituto nazionale biostrutture e biosistemi, Unità di ricerca, Sassari
Publisher:American Society for Microbiology
eISSN:1098-660X
Deposited On:18 Mar 2010 09:30

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