titoli, abstracts, parole chiave >>>
Acidic mammalian chitinase in dry eye conditions

Musumeci, Maria and Aragona, Pasquale and Bellin, Milena and Maugeri, Francesco and Rania, Laura and Bucolo, Claudio and Musumeci, Salvatore (2009) Acidic mammalian chitinase in dry eye conditions. Cornea, Vol. 28 (6), p. 667-672. eISSN 1536-4798. Article.

Full text not available from this repository.

DOI: 10.1097/ICO.0b013e31819bc308

Abstract

Purpose: An acidic mammalian chitinase (AMCase) seems to be implicated in allergic asthma and allergic ocular pathologies. The aim of this work was to investigate the role of AMCase during Sjögren's Syndrome (SS) and Meibomian Gland Dysfunction (MGD) dry eye diseases.
Methods: Six patients with MGD dry eye (20-58 years, median 40) and six patients with dry eye associated to SS (32-60 years, median 47) were enrolled in this study. AMCase activity was measured in tears and AMCase mRNA expression was evaluated by real-time polymerase chain reaction from RNA extracted from epithelial cells of the conjunctiva. Six healthy adult subjects of the same age (34-44 years, median 39) were also studied as the control group.
Results: AMCase activity was significantly increased in patients affected by MGD dry eye (18.54 ± 1.5 nmol/ml/h) and SS dry eye (8.94 ± 1.0 nmol/ml/h) respectively, compared to healthy controls (1.6 ± 0.2 nmol/ml/h). AMCase activity was higher in the tears of subjects with MGD dry eye (P < 0.001). AMCase mRNA was detected in conjunctival epithelial cells and the expression was significantly higher in MGD dry eye than SS dry eye. A significant correlation between AMCase activity in the tears and mRNA in conjunctival epithelial cells was found.
Conclusion: AMCase may be an important marker in the pathogenesis of dry eye, suggesting the potential role of AMCase as a therapeutic target in these frequent pathologies.

Item Type:Article
ID Code:3525
Status:Published
Refereed:Yes
Uncontrolled Keywords:AMCase activity, AMCase mRNA expression, tears, dry eye disease
Subjects:Area 06 - Scienze mediche > MED/38 Pediatria generale e specialistica
Divisions:001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
Publisher:Lippincott Williams & Wilkins
eISSN:1536-4798
Deposited On:25 Jan 2010 15:34

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page