Ortiz, Raúl and Prados Salazar, José Carlos and Melguizo, Consolacion and Rama, Ana Rosa and Segura, Ana and Rodríguez-Serrano, Fernando and Boulaiz, Houria and Hita, Fidel and Martinez-Amat, Antonio and Madeddu, Roberto Beniamino and Ramos, Juan L. and Aranega Jiménez, Antonia (2009) The Cytotoxic activity of the phage E protein suppress the growth of murine B16 melanomas in vitro and in vivo. Journal of Molecular Medicine, Vol. 87 (9), p. 899-911. eISSN 1432-1440. Article.
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Novel treatment modalities, including gene therapy, are needed for patients with advanced melanoma. The E gene from the phage ϕX174 encodes a 91-aa protein which lyses Escherichia coli by formation of a transmembrane tunnel structure. To evaluate whether this E gene has a cytotoxic impact on melanoma cells in vitro and in vivo, and could therefore be used as a new therapeutic strategy for this tumor type, we selected the B16-F10 murine melanoma cell line as a model. We used a nonviral gene delivery approach (pcDNA3.1/E plasmid) to study the inhibition of melanoma cells' proliferation in vitro and direct intratumoral injection of pcDNA3.1/E complexed with jetPEI to deliver E cDNA to rapidly growing murine melanomas, and found that the E gene has both a strong antiproliferative effect in B16-F10 cells in vitro and induces an efficient decrease in melanoma tumor volume in vivo (90% in 15 days).
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