Sanna, Daniela (2010) Quality of in vitro ovine embryo production: lambing rate, body weight and gene expression. Doctoral Thesis.
The objective of this study was to examine the effect of different culture media on in
vitro embryo production in terms of pregnancy rate, lambing rate, lamb body weight and to
compare gene expression in in vivo vs in vitro embryos.
Blastocysts were in vitro produced in different culture media with BSA at different
doses or serum and divided in: A(BSA4), B(BSA8), C(BSA8–HA), D(BSA8-CH) and E
(FBS10) groups. On day 6-7 blastocysts (A, B, C, D groups) were transferred into recipient
ewes either fresh or after vitrification. For gene expression in vitro produced and in vivo
derived (F group) fresh embryos were analyzed by quantitative Real Time PCR. Gene
expression for imprinting (IGF2R, GRB10, UBE2A), embryo development (LAMA1, IL-6,
FGF4), apoptosis (BAX) and methylation (DNMT3A) was analyzed.
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