Sotgia, Salvatore and Carru, Ciriaco and Scanu, Bastianina and Pisanu, Elisabetta and Sanna, Manuela and Pinna, Gérard Aimé and Gaspa, Leonardo and Deiana, Luca and Zinellu, Angelo (2009) Improved rapid assay of plasma uric acid by short-end injection capillary zone electrophoresis. Analytical and Bioanalytical Chemistry, Vol. 395 (8), p. 2577-2582. eISSN 1618-2650. Article.
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A rapid and simple short-end injection capillary zone electrophoresis method was developed for the quantification of plasma uric acid. The separation was performed in an uncoated fused-silica capillary (50 μm ID, 60 cm total length, 10.2 cm effective length) by using as a background electrolyte a 75 mmol/L glycylglycine solution titrated with NaOH 5 mol/L to pH 9.0, a voltage of 28 kV, a cartridge temperature of 15 °C, and direct UV detection at 292 nm. Under optimized conditions, uric acid was determinate in little more than 1 min (1.076 minutes). In order to verify the accuracy of the analysis, urate levels were measured in 543 apparently healthy volunteers by the new assay and our previous method, and the obtained data were compared by Passing–Bablock regression, Bland–Altman test, and a new regression-based approach, which showed a good agreement between two methods.
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