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Improved rapid assay of plasma uric acid by short-end injection capillary zone electrophoresis

Sotgia, Salvatore and Carru, Ciriaco and Scanu, Bastianina and Pisanu, Elisabetta and Sanna, Manuela and Pinna, Gérard Aimé and Gaspa, Leonardo and Deiana, Luca and Zinellu, Angelo (2009) Improved rapid assay of plasma uric acid by short-end injection capillary zone electrophoresis. Analytical and Bioanalytical Chemistry, Vol. 395 (8), p. 2577-2582. eISSN 1618-2650. Article.

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DOI: 10.1007/s00216-009-3171-4

Abstract

A rapid and simple short-end injection capillary zone electrophoresis method was developed for the quantification of plasma uric acid. The separation was performed in an uncoated fused-silica capillary (50 μm ID, 60 cm total length, 10.2 cm effective length) by using as a background electrolyte a 75 mmol/L glycylglycine solution titrated with NaOH 5 mol/L to pH 9.0, a voltage of 28 kV, a cartridge temperature of 15 °C, and direct UV detection at 292 nm. Under optimized conditions, uric acid was determinate in little more than 1 min (1.076 minutes). In order to verify the accuracy of the analysis, urate levels were measured in 543 apparently healthy volunteers by the new assay and our previous method, and the obtained data were compared by Passing–Bablock regression, Bland–Altman test, and a new regression-based approach, which showed a good agreement between two methods.

Item Type:Article
ID Code:3388
Status:Published
Refereed:Yes
Uncontrolled Keywords:Capillary zone electrophoresis, short-end injection, uric acid, method comparison studies
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Area 03 - Scienze chimiche > CHIM/08 Chimica farmaceutica
Divisions:001 Università di Sassari > 02 Centri > Centro di eccellenza interdisc. sviluppo ricerca biotecnologica e studio biodiversità della Sardegna e dell'area mediterranea
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
002 Altri enti e centri di ricerca del Nord Sardegna > National laboratory of the national institute of biostructures and biosystems, Osilo
001 Università di Sassari > 01 Dipartimenti > Farmaco, chimico, tossicologico
Publisher:Springer Berlin / Heidelberg
eISSN:1618-2650
Deposited On:14 Jan 2010 10:50

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