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The Origin of clonal diversity and structure of Populus alba in Sardinia: evidence from nuclear and plastid microsatellite markers

Brundu, Giuseppe Antonio Domenico and Lupi, Renato and Zapelli, Ilaria and Fossati, Tiziana and Patrignani, Giuseppe and Camarda, Ignazio and Sala, Francesco and Castiglione, Stefano (2008) The Origin of clonal diversity and structure of Populus alba in Sardinia: evidence from nuclear and plastid microsatellite markers. Annals of Botany, Vol. 102 (6), p. 997-1006. eISSN 1095-8290. Article.

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DOI: 10.1093/aob/mcn192

Abstract

Background and Aims. Populus alba is a thermophilic forest tree present in the Mediterranean basin. Its habitat is highly fragmented and its distribution range has been subject to long-term human interference, resulting in debate surrounding whether certain populations are native or exotic in origin. In particular, populations from the islands of Corsica and Sardinia are of uncertain origin. While populations of P. alba mainly reproduce sexually, clonal reproduction is also common. The aims of this study were to locate and molecularly characterize the poorly studied island populations of P. alba and compare these with samples from various spatial scales, in order to provide information on the genetic structure and phylogeography of this species. This information will provide evidence on whether the species is native to Sardinia, which is important for the development of conservation strategies.
Methods. DNA extracts were obtained from the following P. alba trees: 159 from Sardinia, 47 from Ticino regional park (northern Italy), 15 acquired from an Italian Germoplasm Bank (IRC; Italian Reference Collection) and 28 from the Mediterranean basin (MB). Genetic polymorphisms were revealed at nuclear and chloroplast DNA (cpDNA) microsatellite loci, both at the island scale (Sardinia) and at broader scales, for comparative assessment of the genetic and genotypic diversity and phylogeography.
Key Results. Based on nuclear microsatellite loci, Sardinian white poplar consists of a small number of genets (26), each of which is represented by several ramets. Despite the uniqueness of the Sardinian haplotypes and the very low value of genetic diversity at the cpDNA level (vK = 0•15), the HT (0•60) and the AR (3•61) values, estimated at the nuclear level for Sardinia, were comparable with those of the other populations and collections.
Conclusions. The uniqueness of the cpDNA haplotypes, the prevalence of clonality and the restricted number of genets recorded suggest that Sardinian white poplar could be a floristic relict of the native flora of the island, which has spread through available habitats on the island mainly by means of vegetative propagation and human activities.

Item Type:Article
ID Code:3117
Status:Published
Refereed:Yes
Uncontrolled Keywords:Populus alba, Sardinia, genets, ramets, phylogeography, native forest species
Subjects:Area 05 - Scienze biologiche > BIO/02 Botanica sistematica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze botaniche, ecologiche e geologiche
Publisher:Oxford University Press on behalf of the Annals of Botany Company
eISSN:1095-8290
Copyright Holders:© The Author 2008
Deposited On:12 Oct 2009 09:32

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