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Genetic variation in five Mediterranean populations of Juniperus phoenicea as revealed by Inter-Simple Sequence Repeat (ISSR) markers

Meloni, Marilena and Perini, Davide and Filigheddu, Rossella Speranza and Binelli, Giorgio (2006) Genetic variation in five Mediterranean populations of Juniperus phoenicea as revealed by Inter-Simple Sequence Repeat (ISSR) markers. Annals of Botany, Vol. 97 (2), p. 299-304. eISSN 1095-8290. Article.

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DOI: 10.1093/aob/mcj024

Abstract

Background and Aims. The assessment of the genetic variability and the identification of isolated populations within a given species represent important information to plan conservation strategies on a genetic basis. In this work, the genetic variability in five natural populations of Juniperus phoenicea, three from Sardinia, one from Cyprus and the last one in the Maritime Alps was analysed by means of ISSRs, on the hypothesis that the latter could have been a refugial one during the last glaciation.
Methods. ISSRs were chosen because of their ability to detect variation without any prior sequence information. The use of three primers yielded 45 reproducible, polymorphic bands, which were utilized to estimate the basic parameters of genetic variability and diversity.
Key Results. All of the populations analysed harboured an adequate amount of genetic variability, with HS = 0•1299. The proportion of genetic diversity between populations has been estimated by GST = 0•12. The three Sardinian populations are separated, as tested by AMOVA, from the Cyprus and the continental ones.
Conclusions. The results indicate that geographical isolation has represented a major barrier to gene flow in Juniperus phoenicea. This work represents a first step towards a full genetic characterization of a conifer from the Mediterranean, a world biodiversity hotspot confronted with climate change, and thus contributes towards the planning of genetics-informed conservation strategies.

Item Type:Article
ID Code:3110
Status:Published
Refereed:Yes
Uncontrolled Keywords:Juniperus phoenicea L, genetic variation, ISSR, conservation, Cupressaceae
Subjects:Area 05 - Scienze biologiche > BIO/03 Botanica ambientale e applicata
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze botaniche, ecologiche e geologiche
Publisher:Oxford University Press on behalf of the Annals of Botany Company
eISSN:1095-8290
Copyright Holders:© The Author 2005
Deposited On:09 Oct 2009 16:33

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