Polymerase chain reaction amplification applied to the direct detection of a 4 bp deletion in the promoter region of the Aγ gene

Polymerase chain reaction amplification applied to the direct detection of a 4 bp deletion in the promoter region of the ^Aγ gene

Manca, Laura and Cocco, Elena and Masala, Bruno Lucio and Gilman, John G. (1990) Polymerase chain reaction amplification applied to the direct detection of a 4 bp deletion in the promoter region of the ^Aγ gene. American Journal of Hematology, Vol. 35 (2), p. 131-133. eISSN 1096-8652. Article.

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DOI: 10.1002/ajh.2830350215

Abstract

We report here the identification of a 4 bp deletion in the ^Aγ^T globin gene promoter by means of Fnu4Hl digestion of DNA amplified by polymerase chain reaction (PCR). This deletion has been previously associated with haplotype II β-thalassemia in Sardinia. This simple, non-radioactive procedure should facilitate the screening of various populations of normal and β-thalassemic subjects for this specific genetic alteration.

Item Type:	Article
ID Code:	3107
Status:	Published
Refereed:	Yes
Uncontrolled Keywords:	PCR, promoter, ^Aγ^T-gene
Subjects:	Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:	001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:	Wiley-Liss
eISSN:	1096-8652
Deposited On:	09 Oct 2009 15:38

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