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Polymerase chain reaction amplification applied to the direct detection of a 4 bp deletion in the promoter region of the Aγ gene

Manca, Laura and Cocco, Elena and Masala, Bruno Lucio and Gilman, John G. (1990) Polymerase chain reaction amplification applied to the direct detection of a 4 bp deletion in the promoter region of the Aγ gene. American Journal of Hematology, Vol. 35 (2), p. 131-133. eISSN 1096-8652. Article.

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DOI: 10.1002/ajh.2830350215

Abstract

We report here the identification of a 4 bp deletion in the AγT globin gene promoter by means of Fnu4Hl digestion of DNA amplified by polymerase chain reaction (PCR). This deletion has been previously associated with haplotype II β-thalassemia in Sardinia. This simple, non-radioactive procedure should facilitate the screening of various populations of normal and β-thalassemic subjects for this specific genetic alteration.

Item Type:Article
ID Code:3107
Status:Published
Refereed:Yes
Uncontrolled Keywords:PCR, promoter, AγT-gene
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Wiley-Liss
eISSN:1096-8652
Deposited On:09 Oct 2009 15:38

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