titoli, abstracts, parole chiave >>>
A Simple approach to the determination of the γ chain composition of F in adult human blood samples

Manca, Laura and Masala, Bruno Lucio (1990) A Simple approach to the determination of the γ chain composition of F in adult human blood samples. Hemoglobin, Vol. 14 (5), p. 517-527. eISSN 1532-432X. Article.

Full text not available from this repository.

Alternative URLs:

Abstract

A procedure for the determination of the γ chain heterogeneity of adult human blood samples with low Hb F has been developed. It consists of the isoelectrofocusing of lysates at a rather high hemoglobin concentration, the isolation of the focused Hb F by elution from the gel, and the analysis of the isolated hemoglobin by reversed phase high performance liquid chromatography for the separation and quantitation of globin chains. Depending on the isoelectrofocusing apparatus, many samples with as little as 1% Hb F may be focused in a single run and the γ chain composition of the recovered hemoglobin (enriched from 15% to 95% in Hb F) easily analyzed by high performance liquid chromatography. The same procedure might be used for the isolation of low level hemoglobin variants for structural studies.

Item Type:Article
ID Code:3106
Status:Published
Refereed:Yes
Uncontrolled Keywords:γ chain composition, adult human blood, hemoglobin variant
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Taylor & Francis
eISSN:1532-432X
Deposited On:09 Oct 2009 15:29

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page