titoli, abstracts, parole chiave >>>
Identification of base substitutions in the promoter regions of the A γ- and G γ-globin genes in A γ- (or G γ-) β+-HPFH heterozygotes using the DNA-amplification-synthetic oligonucleotide procedure

Yang, K. G. and Stoming, Terrance A. and Fei, You-Jun and Liang, Siyuan and Wong, S. C. and Masala, Bruno Lucio and Huang, R. B. and Wei, Z P and Huisman, Titus H. J. (1988) Identification of base substitutions in the promoter regions of the A γ- and G γ-globin genes in A γ- (or G γ-) β+-HPFH heterozygotes using the DNA-amplification-synthetic oligonucleotide procedure. Blood, Vol. 71 (5), p. 1414-1417. eISSN 1528-0020. Article.

Full text not available from this repository.

Alternative URLs:

Abstract

DNA amplification combined with hybridization with 32P-labeled synthetic oligonucleotide probes has been used to identify base substitutions in the 5' promoter region of the A γ-globin gene in members of eleven families from China, Sardinia, Canada, and the United States who had a heterozygosity for the A γ-β+-hereditary persistence of fetal hemoglobin (HPFH), and in members of six black families with a possible G γ-β+-HPFH heterozygosity. All three known A γ types were observed, ie, the British type (-198, T----C), the Chinese type (-196, C----T), and the Green type (-117, G----A); the latter has been found in a black family. Of the six families with G γ-β+-HPFH, three had C----G at -202 and none T----C -175. Conditions for hybridization of amplified DNA with the specific probes are provided and the usefulness of the technique is discussed. The increase in numbers of A γ(G γ)-β+-HPFH heterozygotes with specific base substitutions greatly enhances the probability of a direct correlation between these substitutions and the increase in the production of a specific gamma chain.

Item Type:Article
ID Code:3096
Status:Published
Refereed:Yes
Uncontrolled Keywords:A γ-globin gene, hybridization, DNA amplification, Sardinia
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:American Society of Hematology
eISSN:1528-0020
Deposited On:07 Oct 2009 13:38

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page