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Electrophoretic and chromatographic evidence for allelic polymorphisms in the river buffalo α-globin gene complex

Di Luccia, Aldo and Iannibelli, Luigi and Ferranti, Pasquale and Manca, Laura and Masala, Bruno Lucio and Ferrara, Lino (1991) Electrophoretic and chromatographic evidence for allelic polymorphisms in the river buffalo α-globin gene complex. Biochemical Genetics, Vol. 29 (9-10), p. 421-430. eISSN 1573-4927. Article.

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DOI: 10.1007/BF02399685

Abstract

Isoelectric focusing in the ultranarrow immobilized (7.1–7.5) pH gradient (IPG) of hemoglobin and high-performances liquid chromatography (HPLC) of globin chains were used to investigate Hb polymorphism in Italian river buffalo. Six different phenotypes, each characterized by two or four different Hbs, were detected by IPG, whereas two differentIIα-globin chains were separated from two differentIα-chains by HPLC. Two α-chains (Iα1 andIIα3), and Hbs with similar mobilities (Hb1 andHb3), were associated with the AA Hb phenotype: two α-chains (Iα2 andIIα4), and Hbs with different mobilities (Hb2 and Hb4), were associated with the BB phenotype: two sets of doublet Hbs were associated with the AB phenotype, thus suggesting allelic polymorphisms at the two α loci. An allele at the β locus is responsible for increasing to as many as eight the number of different Hbs, thus further complicating the notable Hb polymorphism of the river buffalo.

Item Type:Article
ID Code:3086
Status:Published
Refereed:Yes
Uncontrolled Keywords:River buffalo hemoglobin, α-globin gene, allelic polymorphism, immobilized pH gradient, high-performance liquid chromatography
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Springer Netherlands
eISSN:1573-4927
Additional Information:Pubblicato online il 1° maggio 2006.
Deposited On:02 Oct 2009 19:09

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