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Evidence for the presence of two different β-globin chains in the hemoglobin of the river buffalo (Bubalus bubalis L.)

Di Luccia, Aldo and Iannibelli, Luigi and Addato, Erminia and Masala, Bruno Lucio and Manca, Laura and Ferrara, Lino (1991) Evidence for the presence of two different β-globin chains in the hemoglobin of the river buffalo (Bubalus bubalis L.). Comparative Biochemistry and Physiology. Part B: Biochemistry and Molecular Biology, Vol. 99 (4), p. 887-892. ISSN 1096-4959. Article.

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DOI: 10.1016/0305-0491(91)90159-B

Abstract

1. 1. Hemoglobin (Hb) of the river buffalo (Bubalus bubalis L.) was studied by employing isoelectric focusing (IEF) in the 6.7–7.7 pH range and by IEF in ultra-narrow immobilized pH gradient (IPG) 7.1–7.5.
2. 2. Three Hb BB phenotypes were identified which were characterized by sets of two or four Hbs with different isoelectric points.
3. 3. These type were called BB, B5B5 and B5, the B5 phenotype showing Hbs with slightly slower mobility.
4. 4. Analysis of constituent globin chains by acid urea polyacrylamide gel electrophoresis in the presence of Triton X-100, provided clear evidence of a novel polymorphism at the β-globin level.
5. 5. Titration curves of β-globins from heterozygous Hb BB5 indicated a single curve thus suggesting the absence of a new charge in all the pH field.
6. 6. A neutral-to-neutral amino acid replacement probably differentiates the two β-globins.

Item Type:Article
ID Code:3082
Status:Published
Refereed:Yes
Uncontrolled Keywords:β-globin chains, acid urea, polymorphism
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Elsevier
ISSN:1096-4959
Additional Information:Pubblicato online il 3 febbraio 2003.
Deposited On:02 Oct 2009 18:26

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