titoli, abstracts, parole chiave >>>
Detection by reversed-phase HPLC of trace amounts of the α113His globin chain in a sheep homozygous for a quadruple α-globin gene chromosome

Vestri, Rita and Pieragostini, Elisa and Manca, Laura and Masala, Bruno Lucio (1988) Detection by reversed-phase HPLC of trace amounts of the α113His globin chain in a sheep homozygous for a quadruple α-globin gene chromosome. International Journal of Biochemistry, Vol. 25 (12), p. 1939-1941. ISSN 1357-2725. Article.

Full text not available from this repository.

DOI: 10.1016/0020-711X(88)90328-X

Abstract

1. 1. In sheep αα113His and αDαα113His globin gene haplotypes, the percentage gene efficiencies, from the 5' to the 3' end, are about 32-18 and 30-14-6, respectively.
2. 2. We previously found that in a αααα homozygote there may be a 1–2% α113His chains, which however were difficult to resolve from ga113Leu chains by CMC chromatography.
3. 3. We report here the experimental conditions which allowed a neat resolution of the 1% α113His chain peak by RP-HPLC.
4. 4. This finding strongly suggests the occurrence of the αDααα113His haplotype where the percentage gene efficiencies are 30-(14-5)-1, supporting the existence of a gradient of decreasing expression in multiple α-globin gene haplotypes.

Item Type:Article
ID Code:3081
Status:Published
Refereed:Yes
Uncontrolled Keywords:Globin gene haplotypes, α-globin gene, sheep homozygous
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Pergamon / Elsevier
ISSN:1357-2725
Additional Information:Pubblicato online il 12 dicembre 2002.
Deposited On:02 Oct 2009 17:57

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page