Abstract

1. 1. In sheep αα^113His and α^Dαα^113His globin gene haplotypes, the percentage gene efficiencies, from the 5' to the 3' end, are about 32-18 and 30-14-6, respectively.
2. 2. We previously found that in a αααα homozygote there may be a 1–2% α^113His chains, which however were difficult to resolve from ga^113Leu chains by CMC chromatography.
3. 3. We report here the experimental conditions which allowed a neat resolution of the 1% α^113His chain peak by RP-HPLC.
4. 4. This finding strongly suggests the occurrence of the α^Dααα^113His haplotype where the percentage gene efficiencies are 30-(14-5)-1, supporting the existence of a gradient of decreasing expression in multiple α-globin gene haplotypes.

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