Abstract

1. 1. The amino acid substitution which characterizes the haemoglobin I variant from sheep has been ascertained using a combination of Fast Atom Bombardment mass spectrometry and protein sequencing.
2. 2. A Ser for Gly substitution at position 13 (10 of the A helix) was found in a polypeptide with the overall sequence of the β^B globin.
3. 3. On the basis of the nucleotide sequence of the β^B-globin gene, a C to T transition occurring on a CpG doublet is considered to be responsible for the amino acid substitution.
4. 4. This represents the first observation of a variant sheep Hb due to a mutation which is rather common in the human genome.
5. 5. Amongst ruminants, serine is normally present at position 13 of goat and sheep ε¹¹ and γ chains and of bovine γ chain which had an independent and more ancient evolutionary origin than the β chains.

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